Ebola Hemorrhagic Fever
Ebola hemorrhagic fever (EHF) is a severe disease caused by several species of Ebolavirus (EBOV), in the family Filoviridae. Prior to 2007, four species of EBOV had been identified, with two (Zaire ebolavirus andSudan ebolavirus) having caused significant disease outbreaks in humans. Outbreaks of EHF are associated with person-to-person transmission after the virus is introduced into humans from a zoonotic reservoir. During outbreaks the virus is commonly transmitted through direct contact with infected persons or their bodily fluids. The onset of EHF is associated with nonspecific signs and symptoms, including fever, myalgias, headache, abdominal pain, nausea, vomiting, and diarrhoea. In the later stages of disease, overt hemorrhage has been reported in up to 50% of cases.
Ebola Bundibugyo virus
The presence of a fifth EBOV virus species,Bundibugyo ebolavirus (BEBOV) was identified after an outbreak of EHF in the Bundibugyo District of western Uganda in 2007.
Ebola virus secreted glycoprotein (sGP)
The virulence of EBOV may be partially attributed to the Ebola virus secreted glycoprotein (sGP), which is the main product transcribed from its GP gene. Ebola virus secreted glycoprotein is secreted from infected cells and can be readily detected in the serum of EBOV-infected hosts. Mature sGP is produced from a precurosor by proteolytic cleavage by host cell proteases such as furin. sGP monomers bind to each other to form a homodimer.
The role of Ebola virus secreted glycoprotein in EBOV pathogenesis is unclear, but is examined in detail by de la Vega et al (2015)
This Ebola virus secreted glycoprotein protein is Ile 33 – Arg 324 (Accession # B8XCN1), fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 34.3 kDa. The protein is expressed in HEK293 cells,. DTT-reduced Protein migrates as 40-55 kDa in SDS-PAGE
****SHIPPING AND STORAGE NOTIFICATION: This recombinant protein has been lyophilised to maximise stability. It is shipped at ambient temperature, and may be stored for up to 1 year at 4C prior to reconstitution. Following reconstitution it should be stored at -80C. Extensive stability tests have shown no negative effects on antigen performance for 7 days of shipping at ambient temperature.