Ebola virus envelope glycoprotein (GP1)
Ebola virus envelope glycoprotein is initially produced as a precursor known as pre-GP, which is cleaved by furin into two subunits, GP1 and GP2, which remain associated through a disulfide linkage between Cys53 of GP1 and Cys609 of GP2. This heterodimer assembles into a 450-kDa trimer at the surface of nascent virions.
The virion-attached GP is critical in the EBOV life cycle, as it is solely responsible for attachment, fusion and entry of target cells. Moreover, GP is responsible for critical pathogenic differences among viral species
The role of Ebola virus envelope glycoprotein in EBOV pathogenesis is unclear, but is examined in detail by Lee and Saphire (2009)
This Ebola virus envelope glycoprotein (GP1) is derived from the GP1 sequence (Accession # AAQ55048.1), expressing Ile33 – Arg501. It is fused with a polyhistidine tag at the C-terminus and has a calculated MW of 51.6kDa. The predicted N-terminus is Ile 33. The protein is expressed in HEK293 cells. DTT-reduced protein migrates as 120-140kDa in SDS-PAGE.
****SHIPPING AND STORAGE NOTIFICATION: This recombinant protein has been lyophilised to maximise stability. It is shipped at ambient temperature, and may be stored for up to 1 year at 4C prior to reconstitution. Following reconstitution it should be stored at -80C. Extensive stability tests have shown no negative effects on antigen performance for 7 days of shipping at ambient temperature.