SDS-PAGE: Purified recombinant Tat clade B (lane 0, molecular weight standard; lane 1, 1µg; lane 2, 2.5µg;) was separated by SDS-PAGE (14% polyacrylamide) and stained with Coomassie Blue.
HIV-1 TAT CLADE B
HIV-1 Tat Clade B protein is recombinantly produced in E. coli and greater than 90% pure.
- Recombinant HIV [HIV-1/Clade B (BH10)] Tat protein is produced in E. coli (Accession number: P69697.1).
- Lyophilized in 0.1% glycerol. Greater than 90% purity.
- Suitable for use in western blotting, SDS Page, Up-Take and Rescue assays. Biologically active protein estimated by Up-Take and Rescue assays (positive to both assays).
- Reacts with anti-Tat antibodies from human, monkey, rabbit and mouse serum.
Immediately after HIV infects a cell, the virion RNA is copied into DNA and the proviral genome is transported to the nucleus and integrated into the host cell genome. Once integrated into the host chromosome, the HIV becomes subject to regulation by cellular transcription factors, as well as its own regulatory proteins. HIV transcription is controlled primarily by the trans-activator protein (Tat). Tat is one of two essential viral regulatory factors (Tat and Rev) for gene expression. Two forms are known, Tat-1 exon (minor form, 72 amino acids) and Tat-2 exon (major form, 86 amino acids) and low levels of both proteins are found in persistently infected cells. Tat has been localized primarily in the nucleolus/nucleus by immunofluorescence. It acts by binding to the TAR RNA element and activating transcription initiation and elongation from the LTR promoter, preventing the 5′ LTR AATAAA polyadenylation signal from causing premature termination of transcription and polyadenylation. It is the first eukaryotic transcription factor known to interact with RNA rather than DNA and may have similarities with prokaryotic anti-termination factors. Tat can be secreted by infected cells and extracellular Tat can be taken up by cells in culture through interactions with heparin sulfate proteoglycans displayed on their surface. Subsequently, Tat reaches their nucleus and can activate a variety of cellular genes including cytokines and cytokine coreceptors.
- Karn, J. Tat, a novel regulator of HIV transcription and latency. HIV sequence database. 2019