Zika Virus NS1 ELISA
The Zika Virus NS1 ELISA assay is a highly sensitive and specific assay suitable for the quantitative detection of Zika Virus NS1 in a range of biological samples.
Zika virus (ZIKV) is a member of the virus family Flaviviridae and the genus Flavivirus, transmitted by Aedes mosquitoes, such as A. aegypti and A. albopictus. Its causes mild fever, joint pain and rash in infected adults but can have life changing affects to the unborn child when the pregnant mother is infected. Zika virus is related to Dengue virus, Yellow Fever, Japanese Encephalitis and West Nile viruses; antibodies (e.g. those produced as a response to infection) may cross-react between these viruses causing problems when diagnosing cases.
The NS1 protein has been reported as being an early marker of viral replication following infection with Zika virus. The protein has been reported to be present in human serum during the early stages of infection (Bosch et al, 2017). This ELISA assay is highly sensitive, with the ability to detect NS1 antigen at levels as low as 5pg/ml.
The Native Antigen Company ELISA assay uses state-of-the-art reagents, including recombinant NS1 protein from our mammalian expression system, and highly specific monoclonal antibodies raised against those antigens. The antibodies show no cross-reactivity with other flaviviruses, including Dengue, West Nile, Yellow Fever, Japanese Encephalitis, Tick-Borne Encephalitis and Usutu virus.
This Zika Virus NS1 ELISA assay offers researchers the opportunity to undertake detailed studies of the level of Zika NS1 protein in infected patients, which may be of real value in studies of the epidemiology of this recently emerged infection. The assay is also suitable to detect NS1 protein in culture, and may therefore be of value in fundamental studies of NS1 expression and function in vitro.
THIS ELISA ASSAY IS FOR RESEARCH USE ONLY. IT IS NOT FOR USE IN DIAGNOSTIC PROCEDURES.