HUMAN IgG1 ANTI-SARS-COV-2 SPIKE (S1) ANTIBODY (HH10)

Recombinant humanized version of the mouse monoclonal antibody MAB12442. Antibody is specific for SARS-CoV-2 spike subunit 1 protein. This antibody has been manufactured for use in ELISA immunoassay development.

PRODUCT DETAILS – HUMAN IgG1 ANTI-SARS-COV-2 SPIKE (S1) ANTIBODY (HH10)

• Recombinant humanized antibody, which is specific for SARS-CoV-2 spike glycoprotein, subunit S1.
• The original monoclonal antibody (MAB12442) also binds to SARS-CoV-2 mutant spike proteins REC31899 (D614G, S477N), REC31900 (D614G, L84I, N439K), REC31901 (D614G, G485R), REC31902 (D614G, E484K) and REC31903 (D614G, V445I, H655Y, E583D), and N-terminal domain (NTD, REC31835). Antibody does not recognize SARS-CoV-2 RBD (Wuhan-Hu-1, UK, Brazilian, Indian Kerala, South African variants), SARS-CoV-2 spike subunit 2 (S2) or spike proteins from SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-OC43, HCoV-229E and HCoV-HKU1.
• Immunogen was recombinant spike subunit 1 protein (REC31806, aa 1-674) expressed in HEK293 cells.
• Suitable for use in ELISA and Western blot.

BACKGROUND

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus induced disease 19 (COVID-19) which emerged in China in late 2019, resulting in a worldwide epidemic (Zhou et al., 2020). SARS-CoV-2 is an enveloped positive-sense single-stranded RNA virus with a number of important structural proteins, including the envelope (E) protein, the membrane (M) protein, the spike (S) protein, and the nucleoprotein (N). The S protein assists in the attachment of the virus to the human cell and comprises intracellular, transmembrane, and extracellular regions. The extracellular region contains the S1 receptor binding subunit (RBD) and the S2 membrane fusion subunit. Generally, following SARS-CoV-2 infection, antibodies appear after 7–14 days and persist for weeks after viral clearance. The most commonly detected antibodies are against the N protein and the S protein. Coronavirus neutralizing antibodies primarily target the trimeric spike (S) glycoproteins on the viral surface (Wang et al., 2020) and can change the course of infection in an infected individual by supporting virus clearance or protecting an uninfected host that is exposed to the virus (Prabakaran et al., 2009). However, the antibody responses against SARS-CoV-2 remain poorly understood (Tang et al., 2020) and better understanding of how the viral coating triggers a healthy immune system’s recognition and neutralisation of the virus is critical for optimisation of diagnostic tests (Petherick, 2020). It has been suggested that spike RBD may be a better target than N for diagnostic tests (Rosadas et al., 2020). The Native Antigens monoclonal antibodies, which are specific for SARS-CoV-2, have been manufactured to meet the need for improved COVID-19 diagnostic assays.

REFERENCES

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