Vaccinia virus was used for smallpox vaccination, but with eradication of smallpox routine vaccination is now longer performed. Recent interest in vaccinia has focused on its possible usage as a vector for immunization against other viruses, with a focus on developing less virulent strains than those used for vaccination against smallpox as vectors.
We offer a recombinant Vaccinia protein that demonstrates biological activity as a Type I IFN receptor.
Vaccinia virus background
Vaccinia virus is a large, complex, enveloped virus belonging to the poxvirus family. Poxviruses are unique among DNA viruses because they replicate only in the cytoplasm of the host cell, outside of the nucleus. Therefore, the large genome is required for encoding various enzymes and proteins involved in viral DNA replication and gene transcription. The close similarity of Vaccinia virus to smallpox virus led to its use as a vaccination to prevent smallpox. Vaccinia virus infection in healthy individuals is generally asymptomatic, or perhaps with a mild fever and rash. It does, however, produce lasting immunity against other pox viruses, including smallpox. Vaccinia virus has therefore been used as a live vaccine against smallpox, and although now not widely used it remains in use for healthcare workers, the US military, and researchers working with pox viruses. The use of the vaccine is associated with very rare, but severe side-effects, primarily in immunocompromised individuals.
We offer recombinant Vaccinia virus B18R protein, which contains two Ig-like C2-type domains and one Ig-like V-type domain. Vaccinia virus B18R exists in a soluble and a membrane-bound form. As a type I IFN receptor, B18R counteracts the antiviral effects of host IFN-alpha/beta. Also, B18R acts as a soluble IFN-alpha receptor and thus inhibits the interaction between host IFN-alpha and its receptor. B18R was recently identified to enable increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs.
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