Human anti-Hepatitis B virus surface antigen HBsAg IgM (M1496), Recombinant

Recombinant human anti-Hepatitis B virus surface antigen HBsAg (M1496) IgM antibody, produced in HEK293 cells and purified by anion exchange chromatography, ammonium sulphate precipitation and dialysis.

PRODUCT DETAILS – Human anti-Hepatitis B virus surface antigen HBsAg IgM (M1496), Recombinant

• Isotype: human IgM
• Clone Number: M1496
• Presented as Liquid in 50mM HEPES, 150mM NaCl, pH8.0

BACKGROUND

Human hepatitis B virus (HBV) is a hepatotropic, partially double-stranded DNA virus capable of causing both acute and chronic infection and is a major global cause of cirrhosis and hepatocellular carcinoma (McMahon BJ, 2009). Hepatitis B surface antigen (HBsAg), the principal envelope protein of HBV, is a key serological marker used to detect active infection, monitor disease progression, and assess treatment response. It is also a major target of the humoral immune response during natural infection and following vaccination (Liang TJ, 2009; Seeger C, Mason WS, 2015). IgM antibodies to HBV antigens—particularly when interpreted alongside HBsAg, anti-HBs, anti-HBc IgM, and HBV DNA—provide important information on recent exposure and acute infection, supporting accurate clinical diagnosis and staging (Hoofnagle JH et al., 1978).

This human monoclonal IgM antibody has been specifically characterized for binding to defined epitopes on hepatitis B surface antigen (HBsAg), enabling sensitive and specific antigen detection in immunoassay formats such as ELISA, chemiluminescent assays, and rapid lateral‑flow tests for HBV screening and diagnostic applications. Its recombinant production supports lot‑to‑lot consistency and makes it suitable for use as a calibrator, control, or capture/detection reagent in assay development and optimization of HBV serology workflows.

REFERENCES

McMahon BJ. The natural history of chronic hepatitis B virus infection. Hepatology. 2009.

Liang TJ. Hepatitis B: the virus and disease. Hepatology. 2009.

Seeger C, Mason WS. Molecular biology of hepatitis B virus infection. Cold Spring Harb Perspect Med. 2015.

Hoofnagle JH et al. Serologic markers of acute and chronic hepatitis B virus infection. Gastroenterology. 1978.

Ecker DM et al. The therapeutic monoclonal antibody market. MAbs. 2015.