Human anti-hepatitis E ORF2 IgG, Recombinant

Anti-Hepatitis E ORF2 (clone M1482) human IgG antibody, produced in HEK293 cells and purified by Protein G affinity chromatography and dialysis.

PRODUCT DETAILS – Human anti-hepatitis E ORF2 IgG, Recombinant

• Isotype: human IgG
• Clone Number: M1482
• Presented as Liquid in DPBS Buffer

BACKGROUND

Hepatitis E virus (HEV) is a non‑enveloped, positive‑sense RNA virus that causes acute hepatitis globally and can lead to severe disease in pregnant and immunocompromised patients (Kamar et al., 2014). The ORF2 gene encodes the capsid protein, which forms the virion shell, contains the main neutralizing epitopes, and is the primary antigen used in current HEV serological assays (Guu et al., 2009; Montpellier and Abravanel, 2021).

Recombinant human anti‑Hepatitis E ORF2 IgG antibodies expressed in Hek293, providing renewable, well‑defined reagents that specifically recognize conformational epitopes on the ORF2 capsid protein and are suitable as capture or detection antibodies, calibrators, or positive controls in ELISA‑ and CLIA‑based HEV assays (Yamashita et al., 2009; Yin et al., 2018). These antibodies, used alongside recombinant ORF2 antigens, support robust HEV serology and the development of sensitive diagnostic tests for HEV exposure and immunity.

REFERENCES

Guu TSY et al. Structure of the hepatitis E virus-like particle suggests mechanisms for virus assembly and receptor binding. Proc Natl Acad Sci U S A. 2009.

Kamar N et al. Hepatitis E virus infection. Clin Microbiol Rev. 2014.

Montpellier C, Abravanel F. The hepatitis E virus Open Reading Frame 2 protein: beyond viral capsid. Viruses. 2021..

Yamashita T et al. Biological and immunological characteristics of recombinant hepatitis E virus ORF2 proteins. Virology. 2009.

Yin X et al. Origin, antigenicity, and function of a secreted form of ORF2 in hepatitis E virus infection. Proc Natl Acad Sci U S A. 2018.