Human anti-Thyroid peroxidase (TPO) IgG (clone 1504), Recombinant

Recombinant human anti-Thyroid peroxidase (TPO) IgG (clone 1504), produced in HEK293 cells and purified by Protein G affinity chromatography and dialysis.

PRODUCT DETAILS – Human anti-Thyroid peroxidase (TPO) IgG (clone 1504), Recombinant

• Isotype: human IgG
• Clone Number: 1504
• Presented as Liquid in 50 mM HEPES, 150 mM NaCl, pH 7.5

BACKGROUND

Anti-thyroid peroxidase (anti-TPO) antibodies are autoantibodies associated with autoimmune thyroid disease, especially Hashimoto’s thyroiditis and, less commonly, Graves’ disease. They target thyroid peroxidase, an enzyme required for thyroid hormone synthesis, and are linked to thyroid dysfunction and an increased risk of hypothyroidism (Franco, J-S. et al. 2013; Aulanni’am, A. et al. 2024).

Anti-TPO antibody testing is commonly performed using automated immunoassays such as ECLIA and CMIA (Manescu, I.B. et al. 2024). These antibodies are also used as diagnostic and predictive markers in thyroid disease (Siriwardhane, T. et al. 2019). Recombinant human anti-TPO antibodies have been described as research reagents for assay development and validation, since patient-derived antibodies are heterogeneous (Rebuffat, S.A. et al. 2010).

REFERENCES

Aulanni’am, A. et al. 2024. Advances in thyroid peroxidase (TPO) and thyroid stimulating hormone receptor (TSHR) biomarkers for autoimmune thyroid diseases. Acta Biochimica Indonesiana, 7(2), 182.

Franco, J-S. et al. 2013. Thyroid disease and autoimmune diseases. In: Autoimmunity: From Bench to Bedside. El Rosario University Press.

Manescu, I.B. et al. 2024. Anti-thyroid peroxidase (TPO) antibodies – Comparative analysis of two automatic methods, ECLIA and CMIA. Revista Română de Medicină de Laborator, 32(1), 33–40.

Rebuffat, S.A. et al. 2010. Human recombinant anti-thyroperoxidase autoantibodies: in vitro cytotoxic activity on papillary thyroid cancer expressing TPO. British Journal of Cancer, 102(5), 852–861.

Siriwardhane, T. et al. 2019. Significance of Anti-TPO as an Early Predictive Marker in Thyroid Disease. Autoimmune Diseases, Article ID 1684074.