AFRICAN SWINE FEVER VIRUS P72 PROTEIN, HIS-TAG (E. COLI)

African Swine Fever virus p72 is a recombinant protein expressed and purified from E. coli cells. Protein is suitable for use in ELISA and SDS-PAGE/Western blot.

PRODUCT DETAILS –  AFRICAN SWINE FEVER VIRUS P72 PROTEIN, HIS-TAG (E.COLI)

• Recombinant ASFV p72 antigen, comprising amino acids 40-315.
• manufactured in E. coli with a 6xHis tag and greater than 95% pure by SDS-PAGE.
• Presented in 25mM Tris Base, 10mM K2CO3.
• Suitable for use in ELISA and Western Blot.

BACKGROUND

African swine fever virus (ASFV) is a large DNA virus and the only member of family Asfarviridae (Dixon et al., 2013). It infects domestic pigs, wild boars, warthogs, and bush pigs, as well as soft ticks (Ornithodoros erraticus), which likely act as a vector. African swine fever (ASF) is characterized by high morbidity and mortality. It is spreading across Europe and in 2018 was introduced into China, leading to high mortality in domestic pigs (Bao et al., 2019). There are no commercial vaccines available for this disease and so control measures are heavily dependent on detection of nucleic acid, antigen, and antibodies.

The genome of ASFV ranges in length from ~170-193 kbp depending on the isolate and contains between 150 and 167 open reading frames (ORFs), which encode 150-200 proteins, with ~50 of them structural, including p30, pp62 and p72 (Jia et al., 2017). For the purpose of serologic detection, p72 antigen is one of the most immunogenic ASFV proteins (Kollnberger et al., 2002), being the major capsid component of viral icosahedrons. It is very important in forming the viral capsid in late stage expression of virus infection (Neilan et al., 2004). The middle portion of the p72 protein contains most epitopes tested by monoclonal antibodies, whilst the C-terminal peptide of p72 is generally used for genotyping ASFV isolates by sequencing (Jia et al., 2017; Heimerman et al., 2018).

REFERENCES

• Bao J, Wang Q, Lin P, et al. Genome comparison of African swine fever virus China/2018/AnhuiXCGQ strain and related European p72 Genotype II strains. Transbound Emerg Dis. 2019;66(3):1167–1176.
• Dixon LK, et al. African swine fever virus replication and genomics. Virus Res 2013;173:3–14.
• Heimerman ME, Murgia MV, Wu P, Lowe AD, Jia W, Rowland RR. Linear epitopes in African swine fever virus p72 recognized by monoclonal antibodies prepared against baculovirus-expressed antigen. J Vet Diagn Invest. 2018;30(3):406–412.
• Jia N, Ou Y, Pejsak Z, Zhang Y, Zhang J. Roles of African Swine Fever Virus Structural Proteins in Viral Infection. J Vet Res. 2017;61(2):135–143.
• Kollnberger SD, et al. Identification of the principal serological immunodeterminants of African swine fever virus by screening a virus cDNA library with antibody. J Gen Virol 2002;83:1331–1342.
• Neilan J.G., Zsak L., Lu Z., Burrage T.G., Kutish G.F., Rock D.L.. Neutralizing antibodies to African swine fever virus proteins p30, p54, and p72 are not sufficient for antibody-mediated protection. Virology. 2004;319:337–342.