HUMAN CALCITONIN PROTEIN, RECOMBINANT

Recombinant human calcitonin, N-terminal His-tag, produced in E.coli and purified by immobilised metal affinity chromatography, ion exchange chromatography and size exclusion

PRODUCT DETAILS – HUMAN CALCITONIN PROTEIN, RECOMBINANT

• Recombinant human calcitonin
• Expressed in Ecoli (Acc. No.: NP_001029124.1)
• Tag: His-tag (N-terminal)
• Presented as liquid in DPBS

BACKGROUND

Human calcitonin (hCT) is a 32-amino acid polypeptide hormone primarily produced by the parafollicular cells (C-cells) of the thyroid gland. It plays a crucial role in calcium and phosphate metabolism by inhibiting osteoclast activity, thereby reducing bone resorption and lowering blood calcium levels. The measurement of calcitonin levels in serum has significant clinical implications, particularly in the diagnosis and management of medullary thyroid carcinoma (MTC), a type of cancer originating from the C-cells of the thyroid (Kiriakopoulos et al., 2022).

The development of sensitive and specific immunoassays for human calcitonin has greatly enhanced our ability to detect and monitor this hormone in various clinical settings. Early radioimmunoassays (RIA) using labeled synthetic human calcitonin and specific antisera demonstrated the presence of calcitonin in normal and pathological conditions, including MTC and chronic renal disease. These assays have been instrumental in understanding the physiological and pathological roles of calcitonin, as well as in the early diagnosis of MTC among high-risk individuals (Tashjian et al., 1970; Deftos, 1971; Rojanasathit & Haddad, 1977).

Recombinant human calcitonin can be used in research to develop new diagnostic tools and improve existing assays, as well as serve as a standard or control to ensure the accuracy and reliability of calcitonin measurements.

REFERENCES

• Kiriakopoulos, A., Giannakis, P., & Menenakos, E. (2022). Calcitonin: Current concepts and differential diagnosis. Therapeutic Advances in Endocrinology and Metabolism, 13.
• Tashjian, A., Howland, B., Melvin, K., & Hill, C. (1970). Immunoassay of human calcitonin. The New England Journal of Medicine, 283(17), 890-895.
• Deftos, L. (1971). Immunoassay for human calcitonin. I. Method. Metabolism: Clinical and Experimental, 20(12), 1122-1128.
• Rojanasathit, S., & Haddad, J. (1977). Human calcitonin radioimmunoassay: Characterization and application. Clinica Chimica Acta; International Journal of Clinical Chemistry, 78(3), 425-437.