Human Sex Hormone – binding globulin, Recombinant

Human sex hormone-binding globulin (SHBG)  expressed in HEK293 cells with Strep II / His-tag and purified on a Strep-tactin column by affinity chromatography, followed by anion exchange chromatography.

PRODUCT DETAILS – Human Sex Hormone-binding globulin, Recombinant

• Accession: P04278
• Tag: His-tag / 2xStrep-tag, C-Terminus
• Expressed and purified from HEK293 cells
• Presented as liquid in DPBS

BACKGROUND

Native sex hormone-binding globulin (SHBG) is a homodimeric glycoprotein of approximately 90 kDa, with each monomer comprising 373 amino acids, two intramolecular disulfide bonds, and glycosylation sites including two biantennary N-linked and one O-linked oligosaccharide (Avvakumov et al., 2001). Its serum concentration is influenced not only by androgens and estrogens but also by thyroid hormones and various metabolic factors. Abnormal SHBG levels are associated with a range of conditions, including hyper- and hypoandrogenism, thyroid and pituitary disorders, liver disease, and hormone-related cancers such as breast and prostate cancer (Thaler et al., 2015).

Recombinant SHBG offers a standardized, well-defined alternative to native protein, eliminating biological variability that can affect assay results. This consistency is particularly valuable for free testosterone measurement, where precise SHBG quantification is critical for accurate calculation. Variability between immunoassay platforms has been shown to significantly impact SHBG measurement and, consequently, free testosterone estimation, with potential for clinical misinterpretation if assay-specific reference ranges are not applied (Adaway et al., 2019).

References

Avvakumov GV, Grishkovskaya I, Muller YA, Hammond GL.(2001) Resolution of the human sex hormone-binding globulin dimer interface and evidence for two steroid-binding sites per homodimer. J Biol Chem.;276(37):34453-7. doi: 10.1074/jbc.M106274200. Epub 2001 Jul 16. PMID: 11457864.

Thaler, M.A., Seifert-Klauss, V., & Luppa, P.B. (2015). The biomarker sex hormone-binding globulin – From established applications to emerging trends in clinical medicine. Best Practice & Research Clinical Endocrinology & Metabolism, 29(5), 749–760.

Adaway J, Keevil B, Miller A, Monaghan PJ, Merrett N, Owen L. Ramifications of variability in sex hormone-binding globulin measurement by different immunoassays on the calculation of free testosterone. Annals of Clinical Biochemistry. 2019;57(1):88-94.