MOUSE ANTI-GIARDIA MONOCLONAL ANTIBODY (E11)

Anti-Giardia monoclonal antibody (E11) recognizes the Cyst-Wall-Protein-1 (CWP1) antigen on the surface of Giardia cysts.

PRODUCT DETAILS – MOUSE ANTI-GIARDIA MONOCLONAL ANTIBODY (E11)

• Mouse anti-Giardia monoclonal antibody (clone E11-3F11E11).
• This antibody is specific for the Cyst-Wall-Protein-1 (CWP1) antigen on the surface of Giardia cysts.
• The antibody will only bind to cysts in the presence of 0.01 to 0.1M calcium chloride. The antibody can be released from cysts by washing in a calcium free solution.
• Buffered in Phosphate Buffered Saline pH7.2, with 0.1% sodium azide.
• Suitable for use in ELISA, IFA, WB.

BACKGROUND

Giardia and Cryptosporidium are both protozoan parasites that produce cysts, which can lead to gut borne infections Giardiasis and Cryptosporidiosis, respectively.  Giardia lamblia (G. lamblia) is an anaerobic, flagellated eukaryotic protozoan, which is a member of the Hexamitidae family of protozoa. Giardia lamblia, also known as Giardia intestinalis or Giardia duodenalis, infects and colonises the small intestine of humans and mammals causing a globally common diarrheal disease known as giardiasis.

Giardia lamblia exists in two forms, as a dormant cyst that infects the small intestine and as a trophozoite, a vegetative form that excysts from the cyst. Trophozoites replicate within the intestine causing the symptoms associated with giardiasis. Both cysts and trophozoites are present in contaminated faeces but trophozoites do not survive for long periods in the environment. G. lamblia cysts are found in soil and water, and on surfaces contaminated by faeces from an infected individual. Giardiasis infection in humans occurs by transmission of dormant G.lamblia cysts via the faecal oral route or through ingestion of contaminated food and water (CDC). Intestinal protozoa are important etiological agents of diarrhea, particularly in children, yet the public health risk they pose is often neglected. Giardia infects approximately 200 million individuals worldwide, and causes acute diarrhea in children under 5 years.

REFERENCES

• Centers for Disease Control and Prevention: Parasites-Giardia