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Mouse Anti-SARS Coronavirus Nucleoprotein Antibody (3864)

$428.34$1,074.82 excl. VAT

Mouse anti SARS Coronavirus nucleoprotein antibody is specific for the nucleocapsid protein of Severe acute respiratory syndrome Coronavirus (SARS-CoV) and also recognises the NP of SARS-CoV-2 (COVID-19) by ELISA. SARS-CoV-2, previously known as the 2019 Novel Coronavirus (2019-nCoV), causes the pandemic COVID-19 disease.

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MAB21425-27_ELISA

Indirect ELISA: mAbs vs. recombinant SARS-CoV-2 (COVID-19) nucleoprotein.
Assays were run as below:

  • Antigen was coated at 2 µg/ml PBS overnight at RT, washed 2X with PBS + 0.3% tween-10 (PBST).
  • Wells blocked with 1% normal goat serum in PBS a minimum of 30 minutes.
  • Antibodies applied at 1 µg/ml PBST for 30 min at RT, washed 2X with PBST. Note: anti Influenza A Nucleoprotein included as a negative control antibody.
  • Goat antibody to mouse IgG+ IgM/HRP conjugate (Jackson Immuno) at 0.5 µg/ml in PBST for 30 minutes at RT. Washed 2X with PBST.
  • TMB added for 1 minute.