Murray Valley encephalitis virus NS1 Protein, Recombinant

Recombinant Recombinant Murray Valley encephalitis virus NS1, expressed in HEK293 cells and purified from culture supernatant. (The protein is predominantly hexameric).

Murray Valley encephalitis virus NS1 Protein, Recombinant

• Accession: APW77688.1
• Strain/Isolate: Canada/human/2011
• Tag: His-Tag, C-Terminus
• Expressed in HEK293
• Presented as liquid in DPBS pH7.4, 0.2μm filter sterilised

BACKGROUND

Murray Valley encephalitis virus (MVEV) is a mosquito‑borne flavivirus endemic to Australia and parts of Papua New Guinea that can cause severe, sometimes fatal, encephalitis in humans during episodic outbreaks (Mackenzie et al., 2017). The non‑structural protein 1 (NS1) of flaviviruses is a secreted glycoprotein that plays roles in viral replication and immune evasion and is a major target of the humoral immune response, making it an important antigen for both diagnostics and vaccine research (Slon Campos et al., 2018).

Recombinant NS1 protein from MVEV can be expressed and purified to provide a well‑defined antigen that does not contain the virion surface envelope (E) protein, thereby avoiding many of the cross‑reactive epitopes associated with E‑directed antibodies to multiple flaviviruses (Young et al., 2000; Henchal & Putnak, 1990). Such recombinant NS1 preparations are suitable for use in serological assays aimed at detecting NS1‑specific antibodies and for experimental immunization studies assessing the contribution of NS1 to protection and pathogenesis in MVEV infection models (Slon Campos et al., 2018; Mackenzie et al., 2017).

REFERENCES

• Henchal, E. A., & Putnak, J. R. (1990). The flavivirus non-structural glycoprotein NS1: structure, maturation and antigenicity. Virology.
• Mackenzie, J. S., et al. (2017). Murray Valley encephalitis virus: its epidemiology, evolution, and prevention. Advances in Virus Research.
• Slon Campos, J. L., et al. (2018). NS1 trifunctional protein: a viral tool for immune evasion and pathogenesis. Nature Reviews Microbiology..
• Young, P. R., et al. (2000). Recombinant flavivirus NS1 proteins as diagnostic antigens. Journal of Virological Methods.