Plasmodium falciparum Etramp 5 protein

Recombinant Plasmodium falciparum Etramp 5, containing an N-terminal GST-tag. Produced in E.coli as soluble protein and purified by affinity chromatography, ion exchange chromatography and dialysis.

PRODUCT DETAILS – Plasmodium falciparum Etramp 5, Recombinant

• Accession: XP_001351869.1
• Tag: GST-tag, N-terminus
• Expressed in E.coli
• Presented as liquid in HBS: 50mM HEPES, 150mM NaCl, pH 8.0

BACKGROUND

Plasmodium falciparum early transcribed membrane protein 5 (Etramp 5) belongs to the ETRAMP family, a group of small, highly charged membrane proteins that are expressed early during the blood stage cycle and localize predominantly to the parasitophorous vacuole membrane (PVM), the interface between the intracellular parasite and the host erythrocyte (Spielmann 2003; Spielmann 2006). ETRAMPs, including Etramp 5, show stage specific and developmentally regulated expression patterns, and their dynamic distribution at the PVM suggests roles in organizing distinct functional membrane domains, parasite nutrient acquisition, and protection of the parasite from host cell factors (Spielmann 2003; Spielmann 2006; Gupta 2020).

Although the precise molecular function of Etramp 5 is still being elucidated, interaction studies have linked ETRAMP family members to host and parasite proteins involved in trafficking and protein quality control, indicating that Etramp 5 may contribute to host–parasite interface remodeling and intracellular survival (Gupta 2020; Sindhe 2019). In parallel, Etramp derived antigens, especially Etramp 5 based constructs, have been incorporated into multiplex serological panels as sensitive markers of recent Plasmodium falciparum exposure, where antibodies to Etramp 5 complement responses to other blood stage antigens in refining sero epidemiological assessment and vaccine related immunoprofiling (Alaerts 2025).

REFERENCES

• Alaerts, M., Vermeulen, M., De Mulder, L., Van Den Bossche, D., Gyselinck, I., Cnops, L., Van Esbroeck, M., & Van Geertruyden, J. P. (2025). A multiplex serological assay to evaluate the antibody responses to Plasmodium falciparum antigens in different epidemiological settings. Vaccines, 13(11), 1091.
• Gupta, I., Joachimiak, L. A., Schröder, G. F., & Przyborski, J. M. (2020). The recognition of proteasomal receptors by Plasmodium proteins. Molecular and Biochemical Parasitology, 236, 111266.
• Sindhe, K. M. V., et al. (2019). Interactions of Plasmodium falciparum ETRAMP14.1 with host proteins (including observations on ETRAMP5 localization in liver‑stage PVM). bioRxiv preprint 775650.
• Spielmann, T., et al. (2003). etramps, a new Plasmodium falciparum gene family coding for early transcribed membrane proteins located at the parasite–host cell interface. Infection and Immunity, 71(10), 5518–5526.
• Spielmann, T., Fergusen, D. J. P., & Beck, H. P. (2006). Organization of ETRAMPs and EXP‑1 at the parasite–host cell interface of Plasmodium falciparum. Molecular Microbiology, 59(3), 779–794.