Recombinant human anti-Chikungunya virus E2 (clone 1488) IgM antibody, produced in HEK293 cells and purified by ammonium sulphate precipitation followed by anion exchange chromatography and dialysis.

PRODUCT DETAILS – Recombinant human anti-Chikungunya virus E2 (clone 1488) IgM antibody

• Isotype: human IgM
• Clone Number: 1488
• Presented as Liquid in 50 mM HEPES, 150 mM NaCl pH 8.0

BACKGROUND

Recombinant human anti-Chikungunya virus E2 IgM antibody binds CHIKV antigens is positioned as a defined anti‑CHIKV research reagent for use in the development or evaluation of diagnostic and serological immunoassays (Guo et al., 2022). It supports CHIKV antigen‑recognition studies in ELISA and related immunoassay formats while keeping claims at an appropriate research‑use level with no clinical performance assertions (Guo et al., 2022).

Because CHIKV testing is stage‑dependent, antigen‑based methods, such as ELISA and rapid tests, are most informative in acute‑phase samples when viremia and antigen levels are higher (Andrew et al., 2022). Conversely, CHIKV‑specific IgM serology becomes more useful after roughly the first week of illness and can persist for months (Islamuddin et al., 2022; Xu et al., 2024). ELISA and related immunoassays remain central to serologic diagnosis, surveillance, and assay validation (Guo et al., 2022). A recombinant monoclonal anti‑CHIKV IgM can therefore support antigen‑binding studies, assay optimization, and comparative format evaluation as a well‑defined CHIKV‑reactive binder for research and test development, without implying clinical utility (Guo et al., 2022).

REFERENCES

Andrew AJ, et al. Diagnostic accuracy of serological tests for the diagnosis of Chikungunya virus infection: A systematic review and meta-analysis. 2022.

Guo M, et al. Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus. 2022.

Islamuddin M, et al. Development of highly sensitive sandwich ELISA for the early-phase diagnosis of Chikungunya virus utilizing rE2-E1 protein. 2022.

Xu C, et al. A portable, integrated, sample-in result-out nucleic acid diagnostic device for rapid and sensitive Chikungunya virus detection. 2024.