Recombinant human S100B protein

Recombinant human S100B protein

Recombinant Human S100B protein with a C-terminal His-tag, produced in Escherichia coli and purified by immobilised metal affinity chromatography and dialysis.

PRODUCT DETAILS – Human S100B, C-terminal His-tag

• Accession: P04271.2
• Expression system: Escherichia coli
• Tag: His-tag (C-terminus)
• Presented as liquid in DPBS

BACKGROUND

S100B is a low-molecular-weight calcium-binding protein of the S100 family, characterised by EF-hand calcium-binding motifs (Donato et al., 2013). It is highly expressed in astrocytes and Schwann cells, where it participates in intracellular calcium signalling, cytoskeletal organisation, and regulation of cell proliferation and differentiation (Donato et al., 2009; Michetti et al., 2019). In the extracellular environment, S100B can act as a signalling molecule with concentration-dependent effects, including interaction with the receptor for advanced glycation end products (RAGE) to influence inflammatory and neurodegenerative pathways (Leclerc et al., 2009; Michetti et al., 2019). Because it is selectively enriched in glial cells and released following cellular stress or injury, S100B is widely used as a biomarker of blood–brain barrier disruption and traumatic brain injury, and it is also studied in the context of neurodegenerative disease (Michetti et al., 2012; Donato et al., 2013). Recombinant S100B is therefore a useful tool for neuroscience research, biomarker assay development, and antibody generation.

REFERENCES

• • Donato, R., Sorci, G., Riuzzi, F. et al. (2009) S100B’s double life: intracellular regulator and extracellular signal. *Biochimica et Biophysica Acta*, 1793(6), pp. 1008–1022.
  • Leclerc, E., Fritz, G., Vetter, S.W. and Heizmann, C.W. (2009) Binding of S100 proteins to RAGE: an update. *Biochimica et Biophysica Acta*, 1793(6), pp. 993–1007.
  • Michetti, F., D’Ambrosi, N., Toesca, A. et al. (2012) The S100B story: from biomarker to active factor in neural injury. *Journal of Neurochemistry*, 120(5), pp. 644–659.
  • Donato, R., Cannon, B.R., Sorci, G. et al. (2013) Functions of S100 proteins. *Current Molecular Medicine*, 13(1), pp. 24–57.
  • Michetti, F., Corvino, V., Geloso, M.C. et al. (2019) The S100B protein in biological fluids: more than a lifelong biomarker of brain distress. *Journal of Neurochemistry*, 148(4), pp. 444–454.