Rheumatoid Factor, Recombinant

The monoclonal recombinant RF is expressed in HEK293 cells as a J-chain–stabilised pentameric human IgM and diluted in human plasma. It shows native-like reactivity and is compatible across major clinical platforms, demonstrating strong recovery in multiple diagnostic systems.

PRODUCT DETAILS – Rheumatoid Factor, Recombinant

• Isotype: human IgM
• Expressed and purified from HEK293 cells
• Presented as liquid in human plasma
• The sample batch is at 6.4mg/ml.

The available batches and their activities are listed below. Please note that if you require a higher activity or concentration, you may wish to consider our Rheumatoid Factor IgM, Recombinant (BSP46000), which is formulated without the addition of plasma. Alternatively, you can contact us to discuss a custom concentration tailored to your platform requirements. Please note that the final pricing will be dependent on the antibody concentration in plasma.

Figure 1. Comparison of our recombinant RF materials and human donor plasma.

BACKGROUND

Rheumatoid arthritis (RA) is a chronic autoimmune disorder marked by joint inflammation and systemic complications, most commonly affecting women and increasing with age [1,6]. Rheumatoid Factor (RF), a group of autoantibodies targeting the Fc region of IgG, is a key biomarker in RA diagnosis and prognosis [2,3,4]. While traditionally sourced from human sera, RF production faces challenges with consistency, supply, and biosafety. Recombinant RF provides a scalable, standardized, and pathogen-free alternative for assay development and quality control in autoimmune diagnostics. Despite its lack of disease specificity, RF remains a core component in diagnostic panels recommended by WHO and ACR [1,5,6].

REFERENCES

1. World Health Organization. Rheumatoid arthritis fact sheet. WHO Website.
2. Maibom-Thomsen SL, Trier NH, Holm BE, et al. Immunoglobulin G structure and rheumatoid factor epitopes. PLoS ONE. 2019;14(6):e0217624.
3. Annals of the Rheumatic Diseases. 2019;78:1667–1668.
4. Aho K, Heliövaara M, Maatela J, et al. Rheumatoid factors antedating clinical rheumatoid arthritis. J Rheumatol. 1991;18(9):1282–1284.
5. Aletaha D, Neogi T, Silman AJ, et al. 2010 Rheumatoid Arthritis Classification Criteria. Ann Rheum Dis. 2010;69(9):1580–1588.
6. National Health Service (NHS) UK. Rheumatoid arthritis diagnosis. https://www.nhs.uk/conditions/rheumatoid-arthritis/diagnosis