WEST NILE NS1 PROTEIN

NAC’s West Nile Virus NS1 protein is produced entirely from human cell lines using state-of-the-art expression techniques, the same approach which NAC used to develop its well received DENV NS1 (4 serotypes) and YFV NS1 proteins.

PRODUCT DETAILS – WEST NILE NS1 PROTEIN

• West Nile Virus NS1 protein (Uniprot Q9Q6P4, strain NY99; aa792-114) produced from HEK293 cells.
• Includes a C-term 6His-tag and greater than 95% purity by SDS-PAGE.
• Presented in PBS, pH7.4.

BACKGROUND

The West Nile Virus (WNV) NS1 protein is a native hexameric presentation. The hexamer is believed to be the biologically active form of NS1 involved in key aspects of West Nile Fever pathogenesis (Kaiser et al., 2017). The resultant WNV NS1 protein is purified to a high degree, is in its native folding state, and possesses all post-translational modifications. This advanced approach results in a product which delivers optimal antigenicity by virtue of its human origin

The West Nile Virus NS1 Protein has been manufactured in response to the unmet need for a highly purified, concentrated protein for use in further vaccine development and serological based diagnostic assays. The detection of the NS1 protein offers much higher fidelity across multiple flaviviruses where cross-reactivity can otherwise pose an issue.

REFERENCES

• Kaiser et al. (2017). Virulence determinants of West Nile virus: how can these be used for vaccine design? Future Virol. 12(5):283-295.

****SHIPPING NOTIFICATION: All NS1 products are shipped at ambient temperature. Extensive stability tests have shown no negative effects on antigen performance for 7 days of shipping. Should customers desire shipment on dry ice, this can be performed for an extra fee, please get in contact with us by phone or email.****