Human Thyrotropin Hormone, Recombinant

Human Thyrotropin hormone, or thyroid-stimulating hormone (TSH), produced in HEK293 cell and purified by affinity chromatography and dialysis. .

PRODUCT DETAILS – Human Thyrotropin hormone, Recombinant

• Accession: P01215 / P01222
• Tag: His-tag / 2xStrep-tag, C-Terminus
• Expressed and purified from HEK293 cells
• Presented as liquid in DPBS

BACKGROUND

Thyrotropin, or thyroid-stimulating hormone (TSH), is a glycoprotein hormone secreted by the anterior pituitary that plays a central role in regulating the endocrine function of the thyroid gland. Structurally, TSH consists of two subunits—alpha and beta—where the alpha subunit is common to other glycoprotein hormones (e.g., LH, FSH), and the beta subunit confers hormonal specificity by binding to TSH receptors on thyroid follicular cells (Szkudlinski et al., 2002). Upon binding, TSH stimulates the synthesis and release of thyroid hormones (T3 and T4), which in turn regulate metabolism, growth, and development. Clinically, serum TSH measurement is a cornerstone in the assessment of thyroid function, often used alongside free T4 and T3 assays for diagnosing hypo- or hyperthyroidism (Pirahanchi et al.,2023). Traditional TSH assays, however, have faced challenges such as cross-reactivity and glycosylation variability. To improve accuracy and standardization, recombinant human TSH has been developed and widely adopted in both diagnostic stimulation tests and thyroid cancer follow-up, offering enhanced assay consistency and safety. Recombinant TSH also eliminates the need for thyroid hormone withdrawal during testing, significantly improving patient quality of life and enabling more sensitive and specific diagnostic procedures (Pacini et al., 2008).

REFERENCES

Szkudlinski, M. W., et al. (2002). Thyroid-stimulating hormone and TSH receptor structure-function relationships. Physiological Reviews, 82(2), 473–502.

Pirahanchi Y, Toro F, Jialal I. Physiology, Thyroid Stimulating Hormone. [Updated 2023 May 1]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan,

Pacini F, Castagna MG. Diagnostic and therapeutic use of recombinant human TSH (rhTSH) in differentiated thyroid cancer. Best Pract Res Clin Endocrinol Metab. 2008 Dec;22(6):1009-21.