Recombinant Human HE4 protein

Recombinant Human HE4 protein

Human HE-4, C-terminal His-tag, produced in HEK293 cells and purified by immobilised metal affinity chromatography and dialysis.

PRODUCT DETAILS –Recombinant Human HE4 (WFDC2), C-terminal His-tag

• Accession: UniProt Q14508
• Expression system: HEK293
• Tag: His-tag (C-terminus)
• Presented as liquid in 50 mM HEPES, pH 8.5, 150 mM NaCl

BACKGROUND

Human epididymis protein 4 (HE4), also known as WFDC2, is a secreted glycoprotein first identified as overexpressed in serous and endometrioid ovarian carcinomas (Drapkin et al., 2005). HE4 is clinically useful as a serum biomarker in ovarian cancer, including for diagnosis and monitoring, and recombinant HE4 provides a defined antigen for studying its biochemical properties and for supporting assay development (Moore et al., 2008; Sokoll et al., 2014).

Recombinant HE4 has been produced in both mammalian and yeast expression systems and shown to be secreted and glycosylated, with glycosylation influencing its biochemical and biological activity (Sokoll et al., 2014). In addition to its diagnostic value, HE4 has been implicated in ovarian cancer progression, and recombinant HE4 has been used in studies exploring tumour-related functions such as proliferation and chemoresistance (Giamougiannis et al., 2023).

REFERENCES

• • Drapkin R, von Horsten HH, Lin Y, et al. Human epididymis protein 4 (HE4) is a secreted glycoprotein that is overexpressed by serous and endometrioid epithelial ovarian carcinomas. Cancer Res. 2005.
  • Moore RG, Brown AK, Miller MC, et al. Utility of a novel serum tumor biomarker HE4 in patients with epithelial ovarian cancer. Gynecol Oncol. 2008.
  • Sokoll LJ, Wiese D, et al. Expression and biochemical characterization of recombinant human epididymis protein 4. 2014.
  • Giamougiannis P, et al. Recent insight about HE4 role in ovarian cancer oncogenesis. 2023.