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BacTrace® Antigens and Antibodies

BacTrace® antigens and antibodies are ideal for developing high performance assays for the detection of bacterial pathogens.

Background

LGC Clinical Diagnostics’ KPL portfolio includes a range of over 800 reagents and kits for immunoassays, immunohistochemistry, infectivity assays and other applications.

BacTrace comprises KPL’s range of native bacterial antigens and matching primary antibodies. Produced in an ISO13485-certified facility, BacTrace bacterial antigens are grown as whole cells in native culture. Cells are then heat-inactivated and validated in infectivity assays, making them safe-to-use, while presenting a broad range of surface epitopes.

BacTrace antibodies are generated by inoculating goats with BacTrace bacterial antigens. The resulting polyclonal sera then undergoes a proprietary affinity purification protocol that preserves target antigen affinity while yielding fractions that are highly specific to the pathogen in question. Combined with downstream cross-reactivity testing, BacTrace antibodies benefit from maximal signal-to-noise ratio, while ensuring defined specificity and lot-to-lot consistency.

 

Anti-E. coli Cross-Reactivity Graph

Figure 1: Lot-to-lot consistency of BacTrace® anti-E. coli O157:H7 polyclonal antibody against panel of bacteria.

Anti-Salmonella CSA-1 Cross-Reactivity Graph

Figure 2: Reactivity of BacTrace® anti-Salmonella polyclonal antibody against panel of Salmonella serotypes and other bacteria.

BacTrace® Products

Our BacTrace® range includes antigens and antibodies for a number of notable food-borne pathogens, as well as other bacterial targets, including:

Pathogen
Antigen
Antibody
Borrelia species
5310-0337
Borrelia burgdorferi
5310-0338
5310-0338
Campylobacter species
5310-0324
Campylobacter jejuni
5370-0011
Clavibacter michiganensis
5310-0318
E. coli O26
5370-0015
5310-0329
E. coli O45
5370-0019
5310-0333
E. coli O91
5370-0021
5310-0335
E. coli O103
5370-0016
5310-0330
E. coli O104
5370-0020
5310-0334
E. coli O111
5370-0014
5310-0328
E. coli O121
5370-0018
5310-0332
E. coli O145
5370-0017
5310-0331
E. coli O157:H7
5370-0013
5310-0327, 5310-0326
Helicobacter pylori
5370-0012
5310-0325
Legionella pneumophila
5370-0005
Listeria species
5310-0319, 5310-0320
Listeria monocytogenes
5370-0010
Renibacterium salmoninarum
5370-0022
5310-0336
Salmonella species
5310-0322, 5310-0323, 5310-0321
Salmonella typhimurium
5370-0002
Shigella species
5370-0003
5310-0310
Staphylococcus aureus
5370-0007
5310-0314
Streptococcus pyogenes (Group A)
5370-0008
5310-0315
Streptococcus agalactiae (Group B)
5370-0009
5310-0316
Vibrio species
5370-0004
5310-0311
Vibrio cholerae
5310-0317
Yersinia species
5310-0313
Yersinia enterolitica
5370-0006

About KPL

KPL was acquired by SeraCare Life Sciences in 2013, which was in turn acquired by LGC in 2018 and is now part of LGC Clinical Diagnostics, alongside The Native Antigen Company.

KPL is a pioneer in the development of large-scale affinity-purified polyclonal antibodies for use in a range of applications. Using proprietary technologies that have been continually refined since the company’s founding in 1979, KPL stands among the industry’s most trusted and reliable sources of high-performance antibodies.