Mouse Anti SARS-CoV-2 Spike (S1) Antibody (FH4)

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Mouse anti SARS-CoV-2 spike (S1) antibody (FH4) is a monoclonal antibody that is specific for the spike subunit 1 (S1) of SARS-CoV-2, the causative agent of COVID-19. Antibody also binds to SARS-CoV-2 mutant spike proteins REC31899 (D614G, S477N), REC31900 (D614G, L84I, N439K), REC31901 (D614G, G485R), REC31902 (D614G, E484K) and REC31903 (D614G, V445I, H655Y, E583D), and N-terminal domain (NTD, REC31835). Antibody does not cross-react in ELISA with SARS-CoV-2 RBD (Wuhan-Hu-1, UK, Brazilian, Indian, South African variants), SARS-CoV-2 spike subunit 2 (S2) or spike proteins from SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-OC43, HCoV-229E and HCoV-HKU1. This antibody has been manufactured for use in ELISA immunoassay development and is suitable for use as a capture or detection antibody in ELISA assays.

Western Blot: 10μl (0.02mg/ml) of SARS-CoV-2 spike S1 protein (REC31806) separated on a Novex 4-12% Bis-Tris gel, alongside a Kaleidoscope marker (BioRad). Primary antibody added at 1 mg/ml (1:1000) and Goat anti mouse secondary antibody (PAB21441HRP) added at 1mg/ml (1:2000).

ELISA: Antigen-down ELISA showing binding of MAB12441 to immobilised SARS-CoV-2 spike subunit 1 (S1) protein (REC31806).

ELISA: Antigen-down ELISA showing binding of MAB12441 to immobilised SARS-CoV-2 virus lysate (NAT41605).

Sandwich ELISA: MAB12441 as capture antibody. SARS-CoV-2 full-length Spike (REC31868) was the capture analyte. Plates were coated with 5µg/ml of MAB12441. Spike protein was added at varying concentrations from 1µg/ml to 0.001ng/ml. Plates were incubated with biotin-labelled MAB12446 detection antibody at 0.9µg/ml.

Sandwich ELISA: MAB12441 as detection antibody. SARS-CoV-2 full-length Spike (REC31868) was the capture analyte. Plates were coated with 5µg/ml of MAB12422. Spike protein was added at varying concentrations from 1µg/ml to 0.001ng/ml. Plates were incubated with biotin-labelled detection antibodies at 1µg/ml.

MOUSE ANTI-SARS-COV-2 SPIKE (S1) ANTIBODY (FH4)

Mouse anti SARS-CoV-2 spike (S1) antibody (FH4) is a monoclonal antibody that is specific for the spike subunit 1 (S1) of SARS-CoV-2, the causative agent of COVID-19. Antibody also binds to SARS-CoV-2 mutant spike proteins REC31899 (D614G, S477N), REC31900 (D614G, L84I, N439K), REC31901 (D614G, G485R), REC31902 (D614G, E484K) and REC31903 (D614G, V445I, H655Y, E583D), and N-terminal domain (NTD, REC31835). Antibody does not cross-react in ELISA with SARS-CoV-2 RBD (Wuhan-Hu-1, UK, Brazilian, Indian, South African variants), SARS-CoV-2 spike subunit 2 (S2) or spike proteins from SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-OC43, HCoV-229E and HCoV-HKU1. This antibody has been manufactured for use in ELISA immunoassay development. Suitable for use as a capture or detection antibody in ELISA assays.

PRODUCT DETAILS – MOUSE ANTI-SARS-COV-2 SPIKE (S1) ANTIBODY (FH4)

Antibody recognizes SARS-CoV-2 spike glycoprotein, subunit S1.
Antibody also binds to SARS-CoV-2 mutant spike proteins REC31899 (D614G, S477N), REC31900 (D614G, L84I, N439K), REC31901 (D614G, G485R), REC31902 (D614G, E484K) and REC31903 (D614G, V445I, H655Y, E583D), and N-terminal domain (NTD, REC31835).
No cross-reactivity in ELISA with SARS-CoV-2 RBD (Wuhan-Hu-1, UK, Brazilian, Indian, South African variants), SARS-CoV-2 spike subunit 2 (S2) or spike proteins from SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-OC43, HCoV-229E and HCoV-HKU1.
Isotype – Mouse IgG1
Immunogen was recombinant spike subunit 1 protein (REC31806, aa 1-674) expressed in HEK293 cells.
Protein G purified from hybridoma culture supernatant.
Suitable for use as capture or detection antibody in ELISA assays. Capture antibody with MAB12446 as detection antibody. Detection antibody with MAB12422 or MAB12446 as capture antibody.

BACKGROUND

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus induced disease 19 (COVID-19) which emerged in China in late 2019, resulting in a worldwide epidemic (Zhou et al., 2020). SARS-CoV-2 is an enveloped positive-sense single-stranded RNA virus with a number of important structural proteins, including the envelope (E) protein, the membrane (M) protein, the spike (S) protein, and the nucleoprotein (N). The S protein assists in the attachment of the virus to the human cell and comprises intracellular, transmembrane, and extracellular regions. The extracellular region contains the S1 receptor binding subunit (RBD) and the S2 membrane fusion subunit. Generally, following SARS-CoV-2 infection, antibodies appear after 7–14 days and persist for weeks after viral clearance. The most commonly detected antibodies are against the N protein and the S protein. Coronavirus neutralizing antibodies primarily target the trimeric spike (S) glycoproteins on the viral surface (Wang et al., 2020) and can change the course of infection in an infected individual by supporting virus clearance or protecting an uninfected host that is exposed to the virus (Prabakaran et al., 2009). However, the antibody responses against SARS-CoV-2 remain poorly understood (Tang et al., 2020) and better understanding of how the viral coating triggers a healthy immune system’s recognition and neutralisation of the virus is critical for optimisation of diagnostic tests (Petherick, 2020). It has been suggested that spike RBD may be a better target than N for diagnostic tests (Rosadas et al., 2020). The Native Antigens monoclonal antibodies, which are specific for SARS-CoV-2, have been manufactured to meet the need for improved COVID-19 diagnostic assays.

REFERENCES

Petherick A. Developing antibody tests for SARS-CoV-2. Lancet. 2020 Apr 4;395(10230):1101-1102.
Prabakaran P, Zhu Z, Xiao X, Biragyn A, Dimitrov AS, Broder CC, Dimitrov DS. Potent human monoclonal antibodies against SARS CoV, Nipah and Hendra viruses. Expert Opin Biol Ther. 2009 Mar;9(3):355-68.
Rosadas C, Randell P, Khan M, McClure MO, Tedder RS. Testing for responses to the wrong SARS-CoV-2 antigen? Lancet. 2020 Sep 5;396(10252):e23.
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