Recombinant Human Cystatin C protein

Recombinant Human Cystatin C protein

Human Cystatin C, produced in HEK293 cells and purified by metal affinity chromatography and dialysis.

PRODUCT DETAILS –Recombinant Human Cystatin C, C-terminal His-tag

• Accession: P01034
• Expression system: HEK293
• Tag: His-tag (C-terminus)
• Presented as liquid in DPBS with 10% glycerol

BACKGROUND

Cystatin C (CstC) is a potent, secreted cysteine protease inhibitor belonging to the type 2 cystatin family and is ubiquitously expressed in human nucleated cells (Grubb et al., 1985; Tsuchida et al., 2015). Clinically, it is best known as a robust serum biomarker for measuring glomerular filtration rate (GFR) to assess kidney function, though its levels are also implicated in various pathological processes, including cardiovascular disease, inflammation, and neurodegeneration (Tsuchida et al., 2015; Xu et al., 2024). Recombinant human Cystatin C serves as a valuable reagent for these clinical and research applications, providing a standardized, renewable source of the protein for calibrating immunoassays and dissecting its inhibitory mechanisms against cathepsins.

Recombinant Cystatin C constructs are produced in various expression systems, including E. coli and mammalian cells, to generate high-purity protein that retains the inhibitory activity against lysosomal proteases (Grubb et al., 1985; Tsuchida et al., 2015). These well-characterized recombinant proteins allow researchers to investigate the multifaceted roles of CstC—such as its involvement in immune modulation, apoptosis, and amyloid deposition—beyond its traditional use as a kidney marker (Tsuchida et al., 2015; Xu et al., 2024). Consequently, recombinant Cystatin C remains a critical tool for improving diagnostic precision and for exploring the broader physiological and pathological functions of this versatile protein.

REFERENCES

• • Grubb AO, et al. Cystatin C as a measure of glomerular filtration rate. Acta Med Scand. 1985.
  • Tsuchida T, et al. Expression, purification, and characterization of human cystatin C. Protein Expr Purif. 2015.
  • Xu Y, et al. Cystatin C is a disease-associated protein subject to multiple regulation. Immunol Cell Biol. 2015.
  • Vilen L, et al. Cystatins: unravelling the biological implications for neuroprotection. Arch Med Sci. 2024.