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Western Blot: Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAB12480 diluted at 1:5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ELISA: Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza B virus nucleoprotein (50 ng). Coated protein was probed with MAB12480 (20000-4.77*10-3 pM). Rabbit IgG antibody (HRP) (1:10000) was used to detect bound primary antibody. EC50: 18.81 Pm.
ELISA: Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using MAB12480 for Capture (5 μg/mL), and MAB12481 for Detection (1 μg/mL).
ICC/IF: MAB12480 detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis. Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green shows Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody MAB12480 diluted at 1:2000.
Influenza B Virus Nucleoprotein Antibody (HL1068)
$781.56 – $3,339.01 excl. VAT
Influenza B virus nucleoprotein antibody is a monoclonal antibody made in rabbit and suitable for the detection of Influenza B virus nucleoprotein. Antibody is suitable for WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA (matched pairs available).
INFLUENZA B VIRUS NUCLEOPROTEIN ANTIBODY (HL1068)
Influenza B virus nucleoprotein antibody is a monoclonal antibody made in rabbit and suitable for the detection of Influenza B virus nucleoprotein.
PRODUCT DETAILS – INFLUENZA B VIRUS NUCLEOPROTEIN ANTIBODY (HL1068)
- Rabbit anti-Influenza B nucleoprotein monoclonal antibody (clone HL1068).
- Recombinant protein encompassing a sequence within the N-terminus region of Influenza B virus Nucleoprotein (B/Taiwan/753/2005).
- Suitable for WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA
- Matched pair antibodies include: Capture: MAB12481 / MAB12482 and Detection: MAB12480 (conjugated to HRP) or Capture: MAB12480 and Detection: MAB12481 / MAB12482
BACKGROUND
Influenza viruses are enveloped, segmented, negative-sense, single-stranded RNA viruses of the Orthomyxoviridae family. There are three genera of influenza virus that are clinically relevant to humans, these are influenza A, B and C. Influenza A and B virus genomes each comprise eight negative-sense, single-stranded viral RNA (vRNA) segments, while influenza C virus has a seven-segment genome. Influenza A viruses circulate worldwide are the most virulent human pathogens among the three influenza types and are the predominant cause of seasonal and pandemic influenza (Bouvier, NM). Influenza B viruses also circulate worldwide and cause seasonal influenza in temperate climates. However, Influenza B viruses are more stable than Influenza A viruses, and are grouped into two lineages B/Yamagata and B/Victoria (CDC).
One of the three core Influenza polypeptides is an RNA-dependent RNA polymerase and a single-strand RNA (ssRNA) binding protein, often referred to as a nucleoprotein (NP). NP encapsidates the virus genome to form a ribonucleoprotein (RNP) particle for the purposes of transcription and packaging. NP is not just the structural component of the virus transcription machinery but performs multiple essential functions throughout the virus life cycle (Portela & Digard, 2002). Influenza A virus RNA segment 5 encodes NP is a polypeptide of 498 amino acids in length; in influenza B and C viruses, the length of the homologous NP polypeptide is 560 and 565 residues, respectively (Londo et al., 1983; Nakada et al., 1984).
REFERENCES
- Bouvier, N.M. and Palese, P. (2008). The Biology of Influenza viruses. Vaccine.26(Suppl 4): D49–D53.
- Center for Disease Control and Prevention: Influenza type A viruses.
- Londo DR, Davis AR, Nayak DP. Complete nucleotide sequence of the nucleoprotein gene of influenza B virus. J Virol. 1983 Sep;47(3):642-8.
- Nakada S, Creager RS, Krystal M, Palese P. Complete nucleotide sequence of the influenza C/California/78 virus nucleoprotein gene. Virus Res. 1984 Sep;1(6):433-41.
- Portela A, Digard P. The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication. J Gen Virol. 2002 Apr;83(Pt 4):723-734.
