Rheumatoid Factor, Recombinant

The monoclonal recombinant RF is expressed in HEK293 cells as a J-chain–stabilised pentameric human IgM and diluted in human plasma. It shows native-like reactivity and is compatible across major clinical platforms, demonstrating strong recovery in multiple diagnostic systems.

PRODUCT DETAILS – Rheumatoid Factor, Recombinant

• Isotype: human IgM
• Expressed and purified from HEK293 cells
• Presented as liquid in human plasma

BACKGROUND

Rheumatoid arthritis (RA) is a chronic autoimmune disorder marked by joint inflammation and systemic complications, most commonly affecting women and increasing with age [1,6]. Rheumatoid Factor (RF), a group of autoantibodies targeting the Fc region of IgG, is a key biomarker in RA diagnosis and prognosis [2,3,4]. While traditionally sourced from human sera, RF production faces challenges with consistency, supply, and biosafety. Recombinant RF provides a scalable, standardized, and pathogen-free alternative for assay development and quality control in autoimmune diagnostics. Despite its lack of disease specificity, RF remains a core component in diagnostic panels recommended by WHO and ACR [1,5,6].

REFERENCES

1. World Health Organization. Rheumatoid arthritis fact sheet. WHO Website.
2. Maibom-Thomsen SL, Trier NH, Holm BE, et al. Immunoglobulin G structure and rheumatoid factor epitopes. PLoS ONE. 2019;14(6):e0217624.
3. Annals of the Rheumatic Diseases. 2019;78:1667–1668.
4. Aho K, Heliövaara M, Maatela J, et al. Rheumatoid factors antedating clinical rheumatoid arthritis. J Rheumatol. 1991;18(9):1282–1284.
5. Aletaha D, Neogi T, Silman AJ, et al. 2010 Rheumatoid Arthritis Classification Criteria. Ann Rheum Dis. 2010;69(9):1580–1588.
6. National Health Service (NHS) UK. Rheumatoid arthritis diagnosis. https://www.nhs.uk/conditions/rheumatoid-arthritis/diagnosis