Human Cytomegalovirus (HCMV)
Human cytomegalovirus (HCMV) is a species of the Cytomegalovirus genus of viruses, which in turn is a member of the viral family known as Herpesviridae or herpesviruses. Infection with CMV is very common, with estimates suggesting that 50-80% of the population in the UK have been infected at some point with CMV. Most cases of CMV infection are asymptomatic, but it can cause illness with symptoms similar to that of Flu or glandular fever.
A significant problem associated with CMV infection results from infection of a pregnant woman, who may then pass the virus on to her newborn child, leading to congenital CMV. A child with CMV infection may experience learning difficulties, and deafness.
Cytomegalovirus glycoprotein B (gB)
Cytomegalovirus glycoprotein B is an important envelope protein of HCMV, acting as a receptor for entry of the virus into fibroblasts. This protein has been the target of vaccines designed to prevent HCMV infection by preventing entry into host cells. However, the HCMV pentameric complex also acts as a receptor for host-cell entry into epithelial and endothelial cells, and vaccine results using gB alone have only shown partial success.
This cytomegalovirus glycoprotein B is derived from the sequence of the Towne strain, (Accession # AAA45920.1) expressing Val23 – Lys700, and is fused with the Fc region of human IgG1 at the C-terminus. The total calculated MW is 104kDa. The furin cleavage site at AA456-459 has been mutated to prevent proteolytic processing during expression. The cytomegalovirus glycoprotein B protein is expressed in HEK293 cells, and DTT-reduced protein migrates as a band of 140-150kDa.
****SHIPPING AND STORAGE NOTIFICATION: This recombinant protein has been lyophilised to maximise stability. It is shipped at ambient temperature, and may be stored for up to 1 year at 4C prior to reconstitution. Following reconstitution it should be stored at -80C. Extensive stability tests have shown no negative effects on antigen performance for 7 days of shipping at ambient temperature.