Preparation of Herpes Simplex Virus 2 antigen:
HSV-2 is propagated in mammalian cells using a protocol optimised for maximum particle yield. At the appropriate time, infected cells are washed in saline then disrupted to release virus particles by freeze thaw and sonication.
Purification and presentation:
Virus particles released from cells are partially purified by low speed centrifugation to remove cell debris. HSV-2 particles are then purified by sucrose density gradients. This process also includes a DNase step to remove cellular DNA and helps to achieve a high purity.
****SHIPPING NOTIFICATION: This product is shipped at ambient temperature. Extensive stability tests have shown no negative effects on antigen performance for 7 days of shipping including several freeze/thaw cycles. Should customers desire shipment on dry ice, this can be performed for an extra fee, please get in contact with us by phone or email.****