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Adenovirus Type 3 Particles, Wild-Type

$1,649.20 excl. VAT

Human adenovirus type 3 particles (wild-type) produced in HEK293 cells and purified by CsCl gradient.

ADENOVIRUS TYPE 3 PARTICLES, WILD-TYPE

This product is a concentrated source of highly-purified human Adenovirus type 3 particles (wild-type) from a lysate of optimally-infected 293 cells. Following double CsCl gradient purification with DNase treatment and dialysis, this Ad3 preparation is of very high quality and minimal lot-to-lot variation. This product has been referenced by Thoma, 2013 and Dyer, 2017.

 

PRODUCT DETAILS – ADENOVIRUS TYPE 3 PARTICLES, WILD-TYPE

  • Wild-type Adenovirus Type 3 particles.
  • Double CsCl gradient purification with DNase treatment and dialysis.
  • Produced in HEK293 cells and stored in pH 7.8 PBS buffer.

 

BACKGROUND

Adenoviruses are medium-sized (80–100 nm), non-enveloped viruses. They have an icosahedral nucleocapsid containing a linear, double-stranded DNA genome of approximately 36 kb (Nermut, 1984). The viral genome is grouped into different transcriptional units, designated early (E1, E2, E3, E4), intermediate, and late. The E1 gene is essential for activation of other viral genes and for viral replication. Deletion of the E1 gene results in viruses that are replication incompetent in normal cells. However, replication-competent viral particles can be produced from E1-deleted viral vectors by providing the E1 gene in trans. The E3 gene is nonessential for either viral replication or infection (Flint, 1999).

Human adenovirus type 3 has a worldwide distribution and is a major cause of febrile respiratory illness, predominantly in children and adolescents, that may or may not involve conjunctivitis.

 

REFERENCES

 

This material is intended for research purposes only, NOT FOR HUMAN USE. Handle material as a category 2 pathogen using category 2 facilities and procedures.

Certificate of analysis
Safety datasheet