Cytomegalovirus glycoprotein B (gB) – Human Fc
CMV glycoprotein B is an important envelope protein of HCMV, acting as a receptor for entry of the virus into fibroblasts. This protein has been the target of vaccines designed to prevent HCMV infection by preventing entry into host cells. However, the HCMV pentameric complex also acts as a receptor for host-cell entry into epithelial and endothelial cells, and vaccine results using gB alone have only shown partial success.
This CMV glycoprotein B is derived from the sequence of the BE/14/2011 strain, and is fused with the Fc region of human IgG1 at the C-terminus via a glycine-serine linker. The total calculated MW is 104kDa. The furin cleavage site at AA457-460 has been mutated to prevent proteolytic processing during expression. The cytomegalovirus glycoprotein B protein is expressed in HEK293 cells, and DTT-reduced protein migrates as a band of 140-150kDa.