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Dengue Virus Serotype 4 NS1 Protein (HEK293)

$497.18$1,822.99 excl. VAT

Recombinant Dengue Virus serotype 4 NS1 protein hexamer, incorporating a C-terminal 6xHis-tag, produced in mammalian HEK293 cells at NS1 protein is available in a choice of two buffers, Tris-Nacl or EPPS-NaCl, both at pH8.5. Tris buffered NS1 protein is suitable for most standard applications. EPPS buffered NS1 is provided for protein conjugation studies.

Citations Available



Dengue Virus Serotype 4 NS1 protein has been manufactured in response to the unmet need for highly purified, concentrated proteins for use in vaccine development (including use as an immunogen) and serological based diagnostic assays. DENV4 NS1 protein is engineered on human cell lines using state-of-the-art expression techniques.

The resultant hexameric presentation is believed to be the biologically active form of NS1 involved in key aspects of dengue pathogenesis. DENV4 NS1 protein is purified to a high degree, are in their native folding state, and possess all post-translational modifications. This advanced approach results in a product which delivers optimal antigenicity due to its human origin.



  • Recombinant Dengue serotype 4 NS1 protein (NCBI Accession Number: P09866.2).
  • DENV4 751-1126 polyprotein amino acids, with a C-terminal His-tag.
  • Sequence Strain is Dominica/814669/1981.
  • Presented as either Tris pH 8.5 or EPPS-NaCl pH 8.5.



With more than one-third of the world’s population living in areas at risk of transmission, dengue infection is a leading cause of illness and death in the tropics and subtropics (ref WHO for Guidelines for Diagnosis, Treatment, Prevention & Control, 2009) As many as 100 million people are infected yearly. Dengue is caused by any one of four related viruses transmitted by mosquitoes. There are no vaccines available to prevent infection with dengue virus.

NAC’s Dengue Virus Serotype 4 NS1 protein has been well received by a wide number of researchers. The proteins are endorsed by the Division of Infectious Disease & Vaccinology at the University of California, Berkeley. Professor Eva Harris states: “For several of our dengue research projects at the University of California, Berkeley, we had been searching for purified DENV NS1 protein from all four serotypes which could offer the advantage of being derived from a mammalian expression system. In our hands, NAC’s DENV NS1 proteins have performed extremely well in a range of applications and have helped advance our research program”.

Based on the same developmental approach as used for DENV4 NS1 protein, NAC also offers NS1 proteins for West Nile Virus and Yellow Fever. Used in combination, the proteins are ideal for determining the integrity of vaccines in development and well as serological based IVD’s.

****SHIPPING NOTIFICATION: All NS1 products are shipped at ambient temperature. Extensive stability tests have shown no negative effects on antigen performance for 7 days of shipping. Should customers desire shipment on dry ice, this can be performed for an extra fee, please get in contact with us by phone or email.****

Certificate of analysis (Tris-NaCl buffer)
Safety datasheet (Tris-NaCl buffer)
Certificate of analysis (EPPS-NaCl buffer)
Safety datasheet (EPPS-NaCl buffer)

Publishing research using our reagents? Please let us know so that we can cite your publication as a reference.

  • Heringer M et al (2017). Dengue type 4 in Rio de Janeiro, Brazil: case characterization following its introduction in an endemic region. BMC Infect Dis. Jun 9;17(1):410. PMID: 28599640