ELISA assay showing specificity of MAB12134 for Dengue Fever Virus Serotype 2 NS1. NS1 antigens from different flaviviruses were coated on an ELISA plate at 0.5ug/ml, and detected with MAB12134 at 0.01ug/ml, visualized with Goat anti-mouse IgG:HRP.
Image by Virology Research Services Ltd
Vero cells were seeded on coverslips and infected with DENV (serotypes 1, 2, 3 and 4) for 48h at MOI 1. Control coverslips consisted of uninfected Vero cells. After fixation with 4% PFA, samples were stained with Mouse anti-Dengue virus NS1 antibody (MAB12134). Antibody was diluted 1:500 and Triton X-100 was used as detergent. Imaging was performed using a Leica SP5 confocal microscope. Immunofluorescence was observed in DENV serotype 2 infected cells, using MAB12134. DENV serotype 2 infected cells (DV2), stained with MAB12134 are shown above (Virology Research Services Ltd).
Dengue virus NS1 antibody (serotype 2) – clone JE1
Mouse anti Dengue virus NS1 antibody (serotype 2) is specific for the NS1 protein of Dengue virus serotype 2. It demonstrates negligible cross-reactivity with NS1 proteins from other Dengue virus serotypes or with other flaviviruses. No cross-reactivity is seen with Chikungunya virus proteins (E1, E2 and C).
This mouse anti Dengue virus NS1 antibody (serotype 2) is suitable for use in direct ELISA and in Western Blot (in non-reducing conditions).
The NS1 protein is a major non-structural protein expressed by the Dengue Virus. The NS1 monomer is a glycosylated protein of approximately 45kD, which associates with lipids and forms a homodimer inside infected cells. It is necessary for viral replication, and is also secreted into the extracellular space as a hexameric lipoprotein particle, which is involved in immune evasion and pathogenesis by interacting with components from both the innate and adaptive immune systems, as well as other host factors. NS1 is one of the major antigenic markers for viral infection with Dengue.
With more than one-third of the world’s population living in areas at risk of transmission, dengue infection is a leading cause of illness and death in the tropics and subtropics (ref WHO for Guidelines for Diagnosis, Treatment, Prevention & Control, 2009) As many as 100 million people are infected yearly. Dengue is caused by any one of four related viruses transmitted by mosquitoes. There are no vaccines available to prevent infection with dengue virus.