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Mouse Anti-Mayaro Virus E1 (M950)

$428.34$1,074.82 excl. VAT

Mouse anti Mayaro virus E1 (M950) protein antibody specific to MAYV. Matched pairs available and suitable for use in sandwich ELISA and Western blot. No cross-reactivity with MAYV E2, Chikungunya virus VLP, E1 and E2, O’nyong’nyong virus VLP and Ross River virus VLP.

Matched Pair

 

Western blot: Antibody is specific for Mayaro virus-like particles (Lane 2; MAYV VLP) containing E1 protein. MAYV VLP contains capsid protein (~29kDa), Spike glycoprotein E2 (~47kDa) and Spike glycoprotein E1 (~48kDa). Antibody did not cross-react with MAYV E2 (Lane 5; human Fc-tag, Lane 6; His-tag), Chikungunya virus VLP (Lane 9; CHIKV VLP), CHIKV E2 (Lane 3; human Fc-tag) or CHIKV E1 (Lane 8; His-tag), O’nyong nyong virus VLP (Lane 4; ONNV VLP) and Ross River virus VLP (Lane 7; RRV VLP).

MAB12324_ELISA

ELISA: Plate was coated with 0.5µg/ml of antigens in 1X DPBS overnight at 2-8ᵒC followed by blocking for 1.5 h using 1%BSA/DPBS, 300µl/well. Plate was washed 3X using Tris wash buffer. 100µl of antibody dilution was added and plate incubated for 2h at RT, 800rpm. Washed plate 3X using Tris wash buffer. Added 100µl of Goat anti mouse IgG HRP antibody (1:5000 dilution) to all the wells and incubated for 1h at RT, 800rpm. Plate was washed 4X using Tris wash buffer and 100µl HKTMB added and incubated for ~2 min. at RT. 100µl 1M HCl was then added to stop the reaction and plate was read at 450nm. Antibody is specific for MAYV VLP in ELISA. It does not cross-react with MAYV E2, Chikungunya virus (CHIKV) VLP, CHIKV E1, CHIKV E2, O’nyong’nyong virus (ONNV) VLP or Ross River virus (RRV) VLP.