Western blot: Antibody is specific for Mayaro virus-like particles (Lane 2; MAYV VLP) containing E1 protein. MAYV VLP contains capsid protein (~29kDa), Spike glycoprotein E2 (~47kDa) and Spike glycoprotein E1 (~48kDa). Antibody did not cross-react with MAYV E2 (Lane 5; human Fc-tag, Lane 6; His-tag), Chikungunya virus VLP (Lane 9; CHIKV VLP), CHIKV E2 (Lane 3; human Fc-tag) or CHIKV E1 (Lane 8; His-tag), O’nyong nyong virus VLP (Lane 4; ONNV VLP) and Ross River virus VLP (Lane 7; RRV VLP).
ELISA: Plate was coated with 0.5µg/ml of antigens in 1X DPBS overnight at 2-8áµ’C followed by blocking for 1.5 h using 1%BSA/DPBS, 300µl/well. Plate was washed 3X using Tris wash buffer. 100µl of antibody dilution was added and plate incubated for 2h at RT, 800rpm. Washed plate 3X using Tris wash buffer. Added 100µl of Goat anti mouse IgG HRP antibody (1:5000 dilution) to all the wells and incubated for 1h at RT, 800rpm. Plate was washed 4X using Tris wash buffer and 100µl HKTMB added and incubated for ~2 min. at RT. 100µl 1M HCl was then added to stop the reaction and plate was read at 450nm. Antibody is specific for MAYV VLP in ELISA. It does not cross-react with MAYV E2, Chikungunya virus (CHIKV) VLP, CHIKV E1, CHIKV E2, O’nyong’nyong virus (ONNV) VLP or Ross River virus (RRV) VLP.
MOUSE ANTI-MAYARO VIRUS E1 (M950)
This mouse anti Mayaro virus E1 (M950) protein antibody is specific to the Acre27 strain of MAYV. Suggested pairs include MAB12326 for capture paired with MAB12324 for detection. We also suggest MAB12324 for capture paired with MAB12325, MAB12326 or MAB12327 for detection.
PRODUCT DETAILS – MOUSE ANTI-MAYARO VIRUS E1 (M950)
- Mouse anti Mayaro virus E1 (IgG1, clone M950). This antibody specific for Mayaro virus (MAYV) E1 and detects MAYV VLP in ELISA and Western blot. It does not cross-react with MAYV E2, Chikungunya virus (CHIKV) VLP, CHIKV E1, CHIKV E2, O’nyong’nyong virus (ONNV) VLP and Ross River virus (RRV) VLP.
- Immunogen used was recombinant Mayaro Virus E1 antigen, Strain Acre27.
- No cross-reactivity in ELISA with MAYV E2, Chikungunya virus (CHIKV) VLP, CHIKV E1, CHIKV E2, O’nyong’nyong virus (ONNV) VLP and Ross River virus (RRV) VLP.
- Purified by Ion Exchange. >90% purity by SDS-PAGE.
- Presented in phosphate buffered saline, pH 7.2 with 0.05% sodium azide.
BACKGROUND
The Mayaro virus (MAYV) capsid is composed of 240 capsid protein copies surrounded by a lipid membrane, through which 80 spike proteins penetrate. Each spike protein is composed of E1-E2 heterodimeric trimers. While E2 is thought to be responsible for binding cellular receptors to mediate entry, the E1 component of the spike protein is involved primarily in cell fusion (Smith et al., 2018). After MAYV attaches to a target cell and is endocytosed, the lower pH of the endosome induces dissociation of the E1-E2 heterodimers to allow trimerization of E1 subunits. These trimers then promote the release of the virion from the endosome and into the cytoplasm for replication.
Mayaro virus (MAYV) is a positive-sense, single-stranded RNA virus, belonging to the Alphavirus genus of the Togaviridae family. It is closely related to other alphaviruses such as Chikungunya, that also produce a dengue fever-like illness that can be accompanied by long-lasting arthritis.
Since MAYV was first characterised in 1954 on the island of Trinidad, it has caused major outbreaks in South American countries. Moreover, reports have suggested that cases of MAYV infection may be higher than previously documented owing to similarities between febrile symptoms of co-circulating Mayaro, Dengue and Chikungunya viruses (CDC).
REFERENCES
- Center for Disease Control and Prevention (CDC): Emerging infectious diseases. Brunini, S et al (2017). High Frequency of Mayaro Virus IgM among Febrile Patients, Central Brazil. Research Letter. Volume 23, Number 6, June.
- Smith et al. (2018). Human Antibody Responses to Emerging Mayaro Virus and Cocirculating Alphavirus Infections Examined by Using Structural Proteins from Nine New and Old World Lineages. Clinical Science and Epidemiology.
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