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SARS-CoV-2 Spike N-Terminal Domain (NTD), Sheep Fc-Tag (HEK293)

$461.90$1,754.60 excl. VAT

Recombinant SARS-CoV-2 Spike protein N-terminal domain (NTD) is manufactured in HEK293 cells and purified to greater than 95% purity from culture supernatant with a sheep Fc-tag. SARS-CoV-2, previously known as the 2019 Novel Coronavirus (2019-nCoV), causes the pandemic COVID-19 disease.

REC31835_SDS-PAGE

SDS-PAGE: REC31835 run on a denaturing SDS-PAGE under reducing conditions, stained with Coomassie.

SARS-COV-2 SPIKE N-TERMINAL DOMAIN (NTD)

Recombinant SARS-CoV-2 Spike protein N-terminal domain (NTD). This domain shows the lowest sequence ID compared to SARS-CoV Spike protein. The protein is produced in HEK293 cells and purified from culture supernatant by Protein G chromatography.

 

PRODUCT DETAILS – SARS-COV-2 SPIKE N-TERMINAL DOMAIN (NTD)

  • SARS-CoV-2 Spike protein N-terminal domain (NTD).
  • Recombinant protein manufactured in HEK293 cells and purified from culture supernatant by Protein G chromatography.
  • Contains Spike protein 1-260, C-terminally tagged with sheep Fc.
  • Presented in Dulbecco’s phosphate buffered saline (DPBS).

 

BACKGROUND

SARS-CoV-2 is a respiratory virus which causes coronavirus disease 2019 (COVID-19). This disease spreads primarily through contact with an infected person via respiratory droplets generated when a person coughs or sneezes, or through droplets of saliva or discharge from the nose. Infection with SARS-CoV-2 can cause mild symptoms including a runny nose, sore throat, cough, and fever.  However, it can be more severe for some people and can lead to pneumonia or breathing difficulties. The elderly, and people with pre-existing medical conditions (such as, diabetes and heart disease) appear to be more vulnerable to becoming severely ill with the virus (WHO, 2020).

The coronavirus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes (Li, 2016). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2, mediating attachment and membrane fusion, respectively. Two major domains in coronavirus S1 have been identified, the N-terminal domain (S1-NTD) and C-terminal domain (S1-CTD). Either or both of these S1 domains can function as a receptor-binding domain (RBD), depending on virus; SARS-CoV and MERS-CoV both use C-domain to bind their receptors (Ou et al., 2020). Angiotensin-converting enzyme 2 (hACE2)21 and human dipeptidyl peptidase 4 (hDPP4)22 have been identifies as the receptors for SARS-CoV and MERS-CoV, respectively. While S proteins of SARS-CoV-2 share about 76% amino acid identities with SARS-CoV, the amino acid sequence of potential RBD of SARS-CoV-2 is only about 74% homologous to that of SARS-CoV. It has been reported that human ACE2 is also the entry receptor of SARS-CoV-2, and that a serine protease is important for SARS-CoV-2 Spike activation (Hoffmann et al., 2020).

 

REFERENCES

  • Hoffmann M, Kleine-Weber H, Schroeder S, et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. 2020;S0092-8674(20)30229-4.
  • Li F. Structure, Function, and Evolution of Coronavirus Spike Proteins. Annu Rev Virol. 2016;3(1):237–261.
  • Ou X, Liu Y, Lei X, et al. Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commun. 2020;11(1):1620.
  • Novel coronavirus (2019-nCoV), World health Organisation (WHO), 2020.

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