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ELISA development

ELISA assay development at The Native Antigen Company is supported by a team with more than 30 years experience in ELISA assay development.

Our ELISA assay development team works closely with the customer to develop a bespoke service to meet their specific requirements. Our aim is always to provide a robust and reproducible assay in agreed timelines, and meeting agreed specifications.

Developing your assay

We provide a bespoke assay development service, offering the flexibility to simply assess the best pair of antibodies for an assay, through to full development of a commercial assay kit.

Where relevant we will include the following parameters:-

  • Intra and Inter Assay precision
  • Normal range and diseased sample range analysis
  • Specificity / cross-reactivity with a range of related targets
  • Linearity
  • LOQ/LOD analysis
  • Parallelism studies

We can support you through the development of your ELISA assay from any stage. If you already have an antigen and antibodies available we will be pleased to use these to identify the best matched pairs, and to determine their performance in ELISA.

However, if your idea is at a much earlier stage we can prepare your antigens, raise your antibodies, and then use these to develop ELISA assays to meet your requirements. Using properly folded and glycosylated proteins from our mammalian expression system gives our development team the edge in ELISA assay development.


Our capabilities
  • Custom antigen production
  • Antibody raising using custom antigen as immunogen (mAbs and pAbs)
  • Antigen/antibody pair optimisation in ELISA
  • Conjugation to enzymes for detection antibodies
  • Plate coating

“We have used The Native Antigen Company to develop a number of new ELISA immunoassays for our product portfolio – their expertise, advice and flexible approach has been invaluable to us.”

– S.S.

” Your service was great, I was in touch with Jackie and she was helpful and quick to respond. We are developing new diagnostic platform, the first step is using recombinant proteins and antibodies sets. So far your products produce very good results. “

– Yehudit M.

Thank you for the information. We really appreciate the work Native Antigen company have done and data you have shared with us as we try to troubleshoot the assay disconnect. We now have an idea where is the disconnect and we will be going back to the drawing board to try and figure out how to proceed

– Ben

Get in Touch With Us

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