Protein Expression Services
The Native Antigen Company produces highly purified, concentrated proteins, for use in a range of research and diagnostic formats. Our senior research scientists hold over thirty years of protein expression experience and use state-of-the-art expression techniques. Our proprietary mammalian, HEK-293 cell line is used for recombinant protein expression to achieve human folding and glycosylation of proteins.
Developing Your Protein
Our mammalian cell line is ideal for the expression of:
- Complex folded and glycosylated proteins
- Viral proteins
- Cancer antigens
For projects where glycosylation is not required, we offer expression in bacterial and insect systems, to ensure we can provide the optimal system for expression of your protein. Our production facilities are highly-scalable, with batch capacities of up to 80L.
The Native Antigen Company’s expression team has decades of experience in the field of recombinant protein production. Our development laboratories are staffed by a core team of PhD molecular biologists and development scientists who are able to execute projects as short as a few days, through to long-term programmes of a year or more. Our protein expression services include:
- Consultation and strategy development with our Director of Research
- DNA design and cloning
- Vector and/or gene optimisation
- Recombinant protein expression in mammalian, bacterial or insect cells
- Protein purification
We understand that protein expression isn’t always straight-forward, so our team its repertoire of cutting-edge technologies and decades of experience in native and recombinant protein production to maximise the success of each project.
From our initial consultation, we develop a project plan to meet your specific requirements, with defined milestones and reporting at every step. We then, typically carry-out a small-scale protein expression test to assess project feasibility before scaling-up to purify larger amounts of your desired protein – minimising your project risk and overall cost.
Zika - development of mature VLPs
Preparation of mature Zika Virus-like particles for EM analysis, developed from our catalogue product for a specific customer requirement
Mutated Dengue Envelope proteins
Development of site-directed mutants of Dengue virus envelope proteins in support of vaccine R&D
Expression and purification of intracellular protein
Preparation of highly purified, bioactive protein, to support oncology clinical trials