GOAT ANTI-ESCHERICHIA COLI O111 SPECIES ANTIBODY

Affinity purified polyclonal antibody to E. coli O111 made in goat (BacTrace®). Isolated from a serum pool of goats immunized with heat-killed whole cells of E. coli serotype O111. The antibody is highly specific for E. coli O111. Each lot is tested to assure specificity and lot-to-lot consistency using an in-house ELISA assay.

PRODUCT DETAILS – GOAT ANTI-ESCHERICHIA COLI O111 SPECIES ANTIBODY

• Part of the BacTrace® range of antigens and antibodies.
• Affinity purified antibody made in goat.
• Isolated from a pool of serum from goats immunized with heat-killed whole cells of E. coli serotype O111.
• This product is designed to react specifically with E. coli O111. Product is tested via ELISA techniques as applicable. Minimal cross-reactivity may exist to other similar species.
• Product is in lyophilized form.
• Each lot is tested to assure specificity and lot-to-lot consistency using an in-house ELISA assay.

BACKGROUND

E. coli was first described in 1885 by Theodor Escherich, an Austrian paediatrician, who called it Bacterium coli commune to reflect its universal occurrence in the faeces of healthy individuals (Escherich 1885). In 1939 the relationship between E. coli and infantile diarrhoea was confirmed (Bray 1945). Serotyping based on the somatic lipopolysaccharide or ‘O’ antigen and the flagella or ‘H’ antigen, helped distinguish the serogroups most likely to be associated with the disease (Kauffmann 1947), which included a set of O serogroups, notably O26, O55 and O111 (Ewing et al. 1963). Shiga toxin-producing Escherichia coli (STEC) are an important cause of haemorrhagic colitis and the diarrhoea-associated form of the haemolytic uraemic syndrome. Of the numerous serotypes of E. coli that have been shown to produce Shiga toxin (Stx), E. coli O157:H7 and E. coli O157:NM (non-motile) are most frequently implicated in human disease (De Boer & Heuvelink, 2000). Human infections with Enterohaemorrhagic Escherichia coli strains (EHEC) as agents of Haemorrhagic Colitis (HC) and Haemolytic Uraemic Syndrome (HUS) are frequently associated with the consumption of EHEC contaminated foodstuffs of different origins. EHEC O26, O103, O111, O118, O121, O145 and O157 strains are responsible for the majority of HC and HUS cases worldwide. Escherichia coli strains of serogroup O111 were the first strains implicated as the main cause of outbreaks of severe gastroenteritis in infant nurseries in the United Kingdom in the 1940s and is now recognized as one of the classic serogroups of enteropathogenic E. coli (EPEC). E. coli O111 strains have been implicated in numerous epidemics of serious enteric disease, including 28% of 50 outbreaks of infantile diarrhea in the United States from 1934 to 1987 (reviewed in Smith et al., 2014).

REFERENCES

• De Boer E, Heuvelink AE. Methods for the detection and isolation of Shiga toxin-producing Escherichia coli. Symp Ser Soc Appl Microbiol. 2000;(29):133S-143S.
• Smith JL, Fratamico PM, Gunther NW 4th. Shiga toxin-producing Escherichia coli. Adv Appl Microbiol. 2014;86:145-97.