Avian Influenza A [HPAI] (H5N1)] Neuraminidase (NA), His-Tag

Recombinant highly pathogenic avian influenza (HPAI) neuraminidase (NA) protein purified from culture supernatant of transfected cells.

PRODUCT DETAILS – Avian Influenza A [HPAI] (H5N1)] Neuraminidase (NA), His-Tag

• Avian Influenza A [HPAI (H5N1)] neuraminidase (Influenza A/Greylag_goose/Scotland/024915/2022)
• Avian Influenza A neuraminidase is expressed in HEK293 cells, and purified from culture supernatant by immobilised metal affinity chromatography and buffer exchange.

BACKGROUND

Avian influenza strains are divided into two types based on their pathogenicity: high pathogenicity (HP) or low pathogenicity (LP). The most well-known HPAI strain, H5N1, was first isolated from a farmed goose in Guangdong Province, China in 1996, and also has low pathogenic strains found in North America. Avian influenza A(H5N8) is an emergent, highly pathogenic avian influenza (HPAI) virus that was first reported in January 2014 and in the same year, HPAIs of subtype H5 caused outbreaks in poultry in Europe, North America and East Asia. In 2015, 2 HPAI strains, HPAI A(H5N2) and HPAI A(H5N8), were identified in both wild birds and poultry in the US and Canada. These 2 strains caused poultry outbreaks across 17 states of the US, affecting more than 11 million birds. Despite widespread outbreaks in poultry and exposure of humans, follow-up studies of contacts did not identify illness in humans. During winter 2016 to 2017, a significant number of European countries, as well as Russia, reported the identification of HPAI A(H5N8) in a variety of wild bird species, including migratory birds. At the beginning of October 2017, 2,781 outbreaks of H5N8 influenza in poultry, wild birds, and captive birds had been reported by the European Union Reference Laboratory for avian influenza. A review of these outbreaks in 2016 to 2017, included information on 524 exposed persons – no human infections were identified by PCR testing. The viruses detected in Europe in 2016 to 2017 and those detected in North America in 2014 to 2015 were all closely related but distinguishable (Adlhoch et al., 2018)

There have been 7 cases of human infection amongst poultry workers at a farm with a known Avian Influenza A(H5N8) outbreak in Russia. Based on available information, the risk of human-to-human transmission remains low and WHO recommendations have not changed as a result of the incident. When avian influenza is circulating in an area, farms and contact with live animals, for example in markets, should be avoided. Precautions must be taken by those working with poultry (WHO).

The current H5N1 clade called 2.3.4.4b, first reported in January 2022, affected millions of commercial poultry and backyard flocks in the United States (CDC). Since then, H5N1 infections have been reported in many previously unreached countries, causing 55 000 wild birds and around 600 sea lions to die in coastal nature preserves in Peru alone (gob.pe)

According to WHO, 873 human infections with influenza A (H5N1) and 458 deaths have been reported globally from 2003 to 25 February 2023. The fatal human case of H5N1 in Cambodia in February 2023 puts avian influenza in the spotlight again. Cambodian health officials, together with WHO, claim that there is no evidence of person-to-person transmission of the virus, although an investigation of the outbreak is ongoing (WHO).

REFERENCES

• Adlhoch C, Dabrera G, Penttinen P, Pebody R; Country Experts. Protective Measures for Humans against Avian Influenza A(H5N8) Outbreaks in 22 European Union/European Economic Area Countries and Israel, 2016 to 2017. Emerg Infect Dis. 2018 Oct;24(10):1-8.
• WHO, Avian influenza A(H5N8) infects humans in Russian Federation, 2021.

• CDC (2023) Update on H5N1 bird flu in Cambodia, Centers for Disease Control and Prevention. Centers for Disease Control and Prevention. Available at: https://www.cdc.gov/flu/avianflu/spotlights/2022-2023/avian-flu-cambodia.htm

• gob.pe (2023) Sernanp Despliega protocolo de monitoreo ante Casos de Aves y Lobos Marinos afectados por influenza aviar en áreas naturales protegidas, Noticias – Servicio Nacional de Áreas Naturales Protegidas por el Estado – Plataforma del Estado Peruano. Available at: https://www.gob.pe/institucion/sernanp/noticias/697084-sernanp-despliega-protocolo-de-monitoreo-ante-casos-de-aves-y-lobos-marinos-afectados-por-influenza-aviar-en-areas-naturales-protegidas (Accessed: March 7, 2023).

• WHO (2023) Avian Influenza A (H5N1) – Cambodia, World Health Organization. World Health Organization. Available at: https://www.who.int/emergencies/disease-outbreak-news/item/2023-DON445