Recombinant Human IgE-Fc Domain

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Recombinant human IgE-Fc domain, a human immunoglobulin epsilon heavy chain constant region (IgE-Fc), manufactured in mammalian cells. Recommended as an immunogen to generate antibodies against human anti-IgE-Fc, a standard/control for human IgE-Fc assays or other human IgE-Fc domain applications.

SDS-PAGE: Representative coomassie-stained SDS-PAGE showing human IgE-Fc domain resolved under non-reducing (NR) and reducing (R) conditions.

ELISA: The IgE Fc domain proteins react with species-specific anti-IgE antibodies, with no cross-reactivity observed.

RECOMBINANT HUMAN IgE-FC DOMAIN

PRODUCT DETAILS – RECOMBINANT HUMAN IgE-FC DOMAIN

Human IgE-Fc domain (V104-G427) with C-terminal 6xHis-tag.
Produced in mammalian HEK293 cells as disulfide-linked homodimer.
Purified by Immobilized Metal Affinity Chromatography and supplied in PBS with preservative (0.02% Proclin 300).
>98%,as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue

BACKGROUND

IgE is distinguished from other antibody classes (IgM, IgD, IgG and IgA) by its ε heavy chains. IgE’s main function is immunity to parasites such as helminths like Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica. It is also utilized during immune defense against certain protozoan parasites such as Plasmodium falciparum. Immunoglobulin E (IgE) is the antibody that plays a central role in the mechanisms of allergic diseases such as asthma, anaphylactic reactions to drugs, bee stings, and antigen preparations used in desensitization immunotherapy (Luker et al., 2019). If you have an allergy, your immune system overreacts to an allergen by producing IgE antibodies. These antibodies travel to cells that release chemicals, causing an allergic reaction. This reaction usually causes symptoms in the nose, lungs, throat, or on the skin. The fragment crystallizable region (Fc region) is the tail region of an antibody that interacts with cell surface receptors called Fc receptors and some proteins of the complement system. This property allows antibodies to activate the immune system. IgE Fc regions contain three heavy chain constant domains (CH domains 2–4) in each polypeptide chain (Sutton et al., 2019).

Recombinant human IgE can be used as reference for IgE concentration measurements in biological samples (e.g. ELISA, FACS).

REFERENCES

Luker et al. A new look at IgE beyond allergies. Version 1. F1000Res. 2019; 8: F1000 Faculty Rev-736.
Sutton et al. IgE Antibodies: From Structure to Function and Clinical Translation. Antibodies (Basel). 2019 Mar; 8(1): 19.