SDS-PAGE: Coomassie-stained SDS-PAGE showing Cytomegalovirus purified antigen.
CYTOMEGALOVIRUS PURIFIED ANTIGEN
Cytomegalovirus purified antigen has been manufactured in response to the in-vitro diagnostics (IVD) industry’s requirement for a cost effective antigen which can demonstrate a high affinity IgG and for IgM. This Cytomegalovirus (CMV) purified antigen when compared to standard lysates reduces the likelihood of cross-reactivity and minimizes lot to lot variation. The product can readily be scaled to meet the need for bulk volumes at significant discounts. CMV purified antigen is offered in a ready to use liquid format.
- Human Cytomegalovirus, strain AD-169 produced in Human Foreskin Fibroblasts (HFF).
- Purified by ultracentrifugationand presented in 0.2 % Triton X-100, 10mM Tris-HCl, 1mM EDTA and 100mM NaCl, pH 7.8.
- Virus particles inactivated by lysis in 0.2% Triton X-100 in STE buffer. The lysate was tested by passage on permissive cells for 2 weeks yielding no CPE.
Cytomegalovirus (CMV, or human betaherpesvirus 5) is a human-specific double-stranded DNA virus, belonging to the Herpesviridae family. CMV is ubiquitous and CMV infection is globally widespread. The virus is transmitted through direct contact with infected bodily fluids, or though contact with virus-infected transplant and transfusion material. Presently, CMV infection is mostly controlled in immune compromised patients by available antiviral drugs, yet it continues to maintain its role as one of the most dangerous infectious agents for an unborn infant. Primary infection is consistently associated with the presence of a virus specific IgM antibody response. Use of NAC’s Cytomegalovirus purified antigen will aid in improving serology based assays for early detection.
NAC offers two further antigens in its CMV range: A Cytomegalovirus gH Pentamer Complex comprised of five viral proteins (gH, gL, UL128, UL130, and UL131) targeted at vaccine research, as well as a Cytomegalovirus cell lysate antigen which contains non-structural CMV proteins as well.