Cytomegalovirus trimer gH/gL/gO Complex His-tag

PRODUCT DETAILS – Human CMV trimer gH/gL/gO Complex His-tag

• Strain AD169
• gH/gL/gO trimeric complex, 6xHis-tag is located at the C-terminus of gH.
• Purified by immobilised metal affinity chromatography and size exclusion chromatography.
• Presented as liquid.

BACKGROUND

Human CMV can cause serious morbidity/mortality in immune compromised individuals such as transplant recipients and HIV patients, and congenital Human CMV infection can lead to birth defects.

It has been shown that both the trimer and pentamer forms of the virus are required for successful Human Cytomegalovirus entry into cells, with trimer primarily interacting with surface receptors, while the pentamer comes into play later in aiding the virus in escaping from endosomes (Liu et al., 2018).

The Pentamer Complex is expressed on all wildtype Human CMV strains but is notably missing from laboratory strains such as AD169. (Freed et al, 2013.). This has placed severe limitations on vaccine development. Receptor-mediated viral entry into endothelial cells requires a functional gH pentameric complex, and this complex is one of the primary targets for antiviral antibodies in infected individuals. NAC’s new recombinant Human CMV gH complex will be key to enabling essential basic and applied research. In addition, the gH complex is expected to provide the basis for a new generation of IVD tests.

The native-like Pentamer and Trimer Complexes are produced in human cells to ensure that all proteins are properly folded and possess their native glycosylation pattern. The presence of all three/five proteins in the purified complex is confirmed by mass spectrometry. The Pentamer and Trimer complexes are offered in ready to use liquid format and is available 100ug and 1mg vials size with the opportunity to purchase bulk quantities.

NAC offers two further antigens in its CMV range: a native high purity antigen which demonstrates a high affinity for both IgG and IgM, ideal for antibody captures assays, and a standard lysate more suited as a serological control.

Cytomegalovirus gH pentamer and trimer are sold as a catalogue items for research use and for use in in vitro diagnostic assay/kits only and as such is without the need for prior agreement with NAC. If the product is intended to be used as a pharmaceutical or as a means to produce a pharmaceutical (including use as an immunogen and for clinical trials), then this must be under prior contractual agreement with NAC.

References

• Freed et al. (2013). Proc Natl Acad Sci U S A.110(51).

• Liu, J. et al. (2018) ‘The human cytomegalovirus trimer and pentamer promote sequential steps in entry into epithelial and endothelial cells at cell surfaces and endosomes’, Journal of Virology, 92(21).