MOUSE ANTI-HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) INTEGRASE (2)
Mouse anti HIV-1 Integrase (2) antibody is specific for HIV-I integrase protein and has been developed for use in ELISA, Western blot and immunofluorescence.
PRODUCT DETAILS – MOUSE ANTI-HUMAN IMMUNODEFICIENCY VIRUS (HIV-1) INTEGRASE (2)
- Mouse anti HIV-1 Integrase (Clone 2). Mouse IgG1,κ.
- Specific for HIV-I integrase protein. No cross-reactivity with non-HIV-1 integrase proteins has been observed.
- Purified from supernatants of hybridoma cell cultures by affinity chromatography.
- Presented in 100mM sodium citrate, 50mM Tris and 0.05% v/v glycerol. Neutral pH.
- For use in ELISA, western blotting and immunofluorescence.
Human immunodeficiency virus (HIV) is a retrovirus (genus Lentivirus) with a single-stranded, positive-sense RNA genome. Upon entry of the target cell, the viral RNA genome is converted to double-stranded DNA by a virally encoded reverse transcriptase that is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integrase allowing the genome to be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latent and the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated to infect other cells. Infection with HIV leads to a condition in which the immune system begins to fail, leading to opportunistic infections.
Integration of viral DNA into a chromosome of the host cell is an essential step in the retroviral life cycle. This process is catalyzed by the viral enzyme integrase (IN) through 3 steps: first step, two nucleotides are removed from the 39 ends of the viral DNA (39-end processing); second step, the recessed 39 ends of the viral DNA are then joined to 59 staggered sites in the target DNA in a concerted cleavage and ligation reaction (DNA joining); last step, integration is completed by repair of the short gaps flanking the viral DNA intermediate and subsequent joining of the 59 ends of viral DNA to the target DNA.
- Chiu and Davies (2004). Structure and function of HIV-1 integrase. Curr Top Med Chem. 4(9):965-77.